ALKBH2 Antibody

說明書

中文名稱：

ALKBH2兔多克隆抗體
貨號：

CSB-PA031205
規格：

￥1100
圖片：
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human fetal liver tissue, Human seminoma tissue, RAW264.7 cells, Primary antibody: CSB-PA031205(ALKBH2 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q6NS38
基因名：

ALKBH2
別名：

2OG Fe(II) oxy DC1 antibody; 9530023G02 antibody; ABH 2 antibody; ABH2 antibody; alkB alkylation repair homolog 2 antibody; ALKB2_HUMAN antibody; ALKBH2 alkB antibody; ALKBH2 antibody; Alkylated DNA repair protein alkB homolog antibody; Alkylated DNA repair protein alkB homolog 2 antibody; Alkylation repair homolog 2 antibody; Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody; AU016977 antibody; DNA oxidative demethylase ALKBH2 antibody; EC 1.14.11. antibody; FLJ99103 antibody; hABH2 antibody; mABH2 antibody; MGC90512 antibody; Oxy DC1 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Fusion protein of Human ALKBH2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
基因功能參考文獻：
1. These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
2. studies reveal the ALKBH2 binding interface of PCNA and indicate that both germline and somatic ALKBH2 variants could have cellular effects on ALKBH2 function in DNA repair. PMID: 26408825
3. It was shown for first time that DNA glycosylase ALKBH2 can repair DNA adduct 1,N2-ethenoguanine. PMID: 25797601
4. ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks in the rDNA genes. PMID: 23972994
5. Overexpression of ALKBH2 is associated with enhanced resistance to temozolomide in glioblastoma. PMID: 23258843
6. ALKBH2 is an upstream molecule of the oncoprotein, MUC1, and regulates cell cycle and EMT, resulting in progression of urothelial carcinomas. PMID: 23279696
7. combination of duplex interrogation and oxidation chemistry allows ALKBH2 to detect and process diverse lesions efficiently and correctly PMID: 22659876
8. The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
9. X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2 PMID: 21510633
10. ABH2 is downregulated in a subset of gastric cancers, and might be involved in the molecular mechanism of gastric cancer through inhibiting the proliferation of gastric cancer cells. PMID: 21155885
11. This work has provided a detailed understanding of the structural features of the single-stranded DNA and double-stranded DNA preferences of ABH2 and ABH3. PMID: 20714506
12. Divergent sequences outside of the active site determine substrate specificities of ABH2. PMID: 20525795
13. Tumour protein (TP53) is directly involved by binding to the promoter of ALKBH2 in mediating photofrin-mediaated photodynamic therapy in U87 glioma cells. PMID: 20661249
14. first crystal structure of ABH2 PMID: 18432238
15. ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions PMID: 18519673
16. Mutations of ABH2 is associated with pediatric brain tumors. PMID: 19290481
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亞細胞定位：

Nucleus. Note=Detected in replication foci during S-phase.
蛋白家族：

AlkB family
組織特異性：

Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
數據庫鏈接：

HGNC: 32487

OMIM: 610602

KEGG: hsa:121642

STRING: 9606.ENSP00000343021

UniGene: Hs.374458

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