S100A8 Monoclonal Antibody

說明書

中文名稱：

S100A8鼠單克隆抗體
貨號：

CSB-MA0206411A0m
規格：

￥1320
圖片：
Immunohistochemical of paraffin-embedded human cervical cancer using CSB-MA0206411A0m at dilution of 1:200

Immunohistochemical of paraffin-embedded human spleen using CSB-MA0206411A0m at dilution of 1:200
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

本產品為針對人源S100A8蛋白研發的高特異性單克隆抗體（CUSABIO貨號：CSB-MA0206411A0m），適用于ELISA和免疫組化（IHC）實驗。S100A8屬于S100鈣結合蛋白家族成員，通常與S100A9形成異源二聚體（Calprotectin），在先天免疫應答中發揮關鍵作用，主要參與調控炎癥反應、細胞遷移及腫瘤微環境重塑，其異常表達與慢性炎癥性疾病、感染性疾病及多種惡性腫瘤的發生發展密切相關。該抗體經嚴格驗證具有優異的人種屬特異性，可精準識別天然構象的S100A8抗原表位，適用于體外檢測組織樣本或細胞培養物中的S100A8蛋白表達分布。在科研領域廣泛應用于炎癥反應機制研究、腫瘤微環境分析、自身免疫性疾病模型構建等方向，特別在類風濕性關節炎、動脈粥樣硬化及結直腸癌等疾病的基礎研究中，為探索S100A8/S100A9復合物的生物學功能及信號通路調控提供可靠工具。本產品采用高純度抗原免疫制備，批次穩定性嚴格質控，確保實驗結果的重復性與準確性。
產品名稱：

mouse anti-Homo sapiens (Human) S100A8 monoclonal antibody
Uniprot No.：

P05109
基因名：

S100A8
別名：

60B8Ag antibody; AI323541 antibody; B8Ag antibody; BEE11 antibody; CAGA antibody; Calgranulin-A antibody; Calprotectin L1L subunit antibody; Calprotectin; included antibody; CFAG antibody; CGLA antibody; Chemotactic cytokine CP-10 antibody; CP-10 antibody; Cystic fibrosis antigen antibody; L1Ag antibody; Leukocyte L1 complex light chain antibody; MA387 antibody; MIF antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; Myeloid-related protein 8 antibody; Neutrophil cytosolic 7 kDa protein antibody; NIF antibody; p8 antibody; Pro-inflammatory S100 cytokine antibody; Protein S100-A8 antibody; S100 calcium binding protein A8 (calgranulin A) antibody; S100 calcium binding protein A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S100A8/S100A9 complex; included antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody
宿主：

mouse
反應種屬：

Human
免疫原：

Recombinant Human S100A8
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

monoclonal
抗體亞型：

IgG1
純化方式：

>95%,protein G purifed
克隆號：

1A1C1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

liquid
應用范圍：

ELISA,IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:500

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.; (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.
基因功能參考文獻：
1. Study found that that the co-stimulation with both advanced glycation end-products and Porphyromonas gingivalis LPS increased S100A8 and S100A9 mRNA expression in gingival epithelial cells. These results demonstrated that the upregulation of calprotectin in gingival epithelial cells may primarily function intracellularly in this tissue and may contribute to the pathological process of periodontitis. PMID: 28771806
2. Our results showed that S100A8/A9, absent or present at very low level in skin biopsies from healthy subjects, is dramatically upregulated in each epidermal layer from psoriatic patients. PMID: 29605872
3. The results suggest that the calcium-binding proteins S100A8 and S100A9 in myeloid cells have an immune regulatory effect. PMID: 29933628
4. Median MRP8/14 levels were higher in patients with Takayasu arteritis. PMID: 29196802
5. S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes. PMID: 29595187
6. Wide intra- and interindividual differences in calprotectin concentrations in the meconium may reflect intestinal inflammation associated with the fetal adaptation to life outside the uterus. PMID: 28463850
7. Salivary calprotectin levels were significantly higher in RAS [Recurrent aphthous stomatitis] group (23.72 +/- 4.28 mg/L) compared to the HC [Healthy Controls] (21.59 +/- 4.27 mg/L) (P = 0.013). No significant relationship was found between calprotectin levels and age or gender in both groups (P >0.05). PMID: 29519972
8. the levels of S100A8 and S100A9 in the sera, and calprotectin levels in the sera and urines of bladder cancer patients, were determined. PMID: 28609200
9. MRP-8/14 concentration increases in coronary artery blood in association with thrombus formation in acute coronary syndrome. PMID: 28986057
10. ANGPTL6, S100A8 and S100A9 are overexpressed in skin of psoriasis patients versus zero expression in controls. PMID: 27698489
11. Elevated breast cancer cell and stromal S1008 protein expression are significant indicators of poorer outcomes in early stage breast cancer patients. Evaluation of S100A8 protein expression may provide additional prognostic information beyond traditional breast cancer prognostic biomarkers. PMID: 28717852
12. S100A8 was required for laser-induced new collagen synthesis in skin cells. PMID: 29270708
13. S100A8 methylation has a role in diagnosis and prognosis of hepatocellular carcinoma PMID: 27462864
14. This study demonstrated that S100A8 was detected in the serums of Alzheimer disease patients PMID: 27911324
15. Studies indicate S100A8 as the most overexpressed gene, and the cell cycle pathway as the most promising biomarker and therapeutic target for relapsed childhood B-acute lymphoblastic leukemia (B-ALL). PMID: 28183295
16. Low S100A8 expression is associated with head and neck neoplasms. PMID: 26883112
17. Report role of fecal calprotectin assay in the diagnosis and management of inflammatory bowel disease. PMID: 28735301
18. S100A8 and S100A9 aggravate Coxsackievirus B3-induced myocarditis and might serve as therapeutic targets in inflammatory cardiomyopathies. PMID: 29158436
19. Data suggest that fecal calprotectin may be a potential biomarker to identify patients with ankylosing spondylitis (AS) at risk of developing inflammatory bowel disease (IBD). PMID: 28148281
20. Elevated S100A8 and S100A9 gene expression in SP-infected HMEECs and in the middle ear mucosa of OM, minor co-localized with neutrophil markers suggests that middle ear epithelial cell secretion of S100A8 and S100A9 may play a role in the pathogenesis of recurrent and chronic OM PMID: 28964306
21. results of this study support an additional role of calprotectin in assessing inflammatory activity in patients with RA PMID: 28832684
22. Data show that E3 ubiquitin ligase HRD1 (HRD1) decreased the protein level of S100A8 through ubiquitination. PMID: 28423597
23. High S100A8 expression is associated with glioblastoma. PMID: 27006175
24. This study implicates S100A8 and S100A9 as important mediators of tumor cell aggressiveness, and highlights the therapeutic potential of S100A8 and S100A9 for interference of metastasis. PMID: 27086923
25. Data indicate a statistical correlation between fecal calprotectin (FC) and gastrointestinal graft-versus-host disease (GvHD). PMID: 28293713
26. Perinatal alarmins S100A8 and S100A9 specifically altered MyD88-dependent proinflammatory gene programs. S100 programming prevented hyperinflammatory responses without impairing pathogen defense. TRIF-adaptor-dependent regulatory genes remained unaffected by perinatal S100 programming and responded strongly to lipopolysaccharide, but were barely expressed. PMID: 28459433
27. Expression of S100A8, S100A9 and S100A12 is modulated by eicosapentaenoic acid and docosahexaenoic acid during Inflammation in adipose tissue and mononuclear cells. PMID: 28125622
28. High S100A8 expression is associated with lung metastasis in malignant melanoma. PMID: 27151304
29. Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID: 27936243
30. S100A8 and S100A9 proteins in concentrations secreted by CD34(-)/CD31(+) circulating angiogenic cells (CACs) with impaired function reduce endothelial cell capillary-like network formation. These effects appear to be mediated by Toll-like receptor 4 and are absent with S100A8 and S100A9 in concentrations secreted by healthy CD34(-)/CD31(+) CACs. PMID: 27793853
31. MRP8 elevated serum levels in patients with Behcet's disease indicate MRP8 role in the pathogenetic mechanisms of Behcet's disease. PMID: 25471894
32. High baseline serum MRP8/14 levels are associated with lower survival in Chinese peritoneal dialysis patients. PMID: 22699162
33. In acute coronary syndromes, MRP8 and MRP8/14 complex are specific ligands of TLR4, which induce the release of TNFalpha and probably other pro-inflammatory agents from monocytes PMID: 21782178
34. study revealed a novel role of MRP8/14 in the autophagy-mediated elimination of intracellular BCG by promoting reactive oxygen species generation PMID: 25312864
35. MRP8 has a crucial role in stimulating IL-6 expression by rheumatoid arthritis fibroblast-like synoviocytes. PMID: 23619188
36. decreased pretreatment levels of myeloid-related protein-8/14 and C-reactive protein may have a role in poor adherence to treatment with tumor necrosis factor inhibitors in juvenile idiopathic arthritis PMID: 27562034
37. MRP8/14 can activate cells by binding to TLR4, leading to the release of proinflammatory cytokines. These mediators can lead to increased disease activity in enthesitis-related arthritis. PMID: 24231443
38. Fibronectin adhesive capacity and integrin expression of monocytes of type 1 and type 2 diabetic patients is related to the subjects' serum levels of MRP-8. PMID: 15277376
39. Excessive release of cytotoxic MRP8/MRP14 by activated phagocytes might therefore present an important molecular pathomechanism contributing to endothelial damage during vasculitis and other inflammatory diseases PMID: 17095618
40. MRP8 expression is related to the pathological stage of laryngeal squamous cell carcinoma. PMID: 22256739
41. MRP8/14 in ankylosing spondylitis is associated with peripheral arthritis and active disease PMID: 27738838
42. Circulating MRP-8/14 is associated with TX-dependent platelet activation in ACS, even during low-dose aspirin treatment, suggesting a contribution of residual TX to MRP-8/14 shedding, which may further amplify platelet activation. PMID: 25037196
43. Phosphorylation inhibits tubulin polymerization in microtubules. PMID: 15331440
44. Data suggest that tissue infiltrating macrophages secreting MRP8/14 may contribute to myositis, by driving the local production of cytokines directly from muscle. PMID: 24286299
45. calprotectin induces IL-6 and MCP-1 production in Human Gingival Fibroblasts via TLR4 signaling that involves MAPK and NF-kappaB, resulting in the progression of periodontitis. PMID: 27925202
46. Findings suggest that S100A8/A9 may be involved in the inflammatory response with induction of proinflammatory cytokines and may serve as a clinicopathological marker for disease activity in AOSD. PMID: 27537874
47. Peripheral blood levels of S100A8 and TRAF6 in SAE patients were elevated and might be related to the severity of SAE and predict the outcome of SAE. PMID: 27411454
48. Low S100A8/A9 levels may contribute to poor wound healing in colonized chronic wounds with striking difference between diabetic foot ulcers and venous leg ulcers. PMID: 27084691
49. Suggest that, in individuals with lichen planus, S100A8 may exert distinct immunomodulatory and cytotoxicity functions. PMID: 26632637
50. Serum calprotectin was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. It was higher in seropositive than in seronegative patients. PMID: 26767827
顯示更多

收起更多
亞細胞定位：

Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.
蛋白家族：

S-100 family
組織特異性：

Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes
數據庫鏈接：

HGNC: 10498

OMIM: 123885

KEGG: hsa:6279

STRING: 9606.ENSP00000357721

UniGene: Hs.416073

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
VIM Recombinant Monoclonal Antibody

Applications: ELISA, IHC, IF

Species Reactivity: Human