KRT8 Monoclonal Antibody

說明書

中文名稱：

KRT8鼠單克隆抗體
貨號：

CSB-MA999890
規格：

￥1320
圖片：
IHC image of CSB-MA999890 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

KRT8單克隆抗體（CUSABIO貨號：CSB-MA999890）是針對人類角蛋白8（Keratin 8）靶點開發的科研用試劑，采用小鼠雜交瘤技術制備，屬于IgG1 kappa亞型。KRT8作為II型中間絲角蛋白家族成員，與KRT18形成異源二聚體，廣泛分布于單層上皮細胞及腺上皮來源的腫瘤組織中，在腺癌、導管癌等惡性腫瘤的細胞骨架維持和分化調控中具有重要研究價值。本產品經過ELISA和免疫組化（IHC）雙重驗證，適用于體外實驗中對KRT8蛋白表達的定性與半定量分析，尤其可用于消化道腫瘤、乳腺癌及肝癌等實體瘤研究的組織切片標記、細胞形態學觀察及分子機制探索。該抗體具有高批次穩定性，可直接用于常規免疫檢測體系，為病理學、腫瘤生物學及細胞分化研究提供可靠工具。通過特異性識別KRT8抗原表位，可幫助科研人員精準定位上皮源性細胞，在癌癥分子分型、轉移機制及藥物靶點篩選等領域具有廣泛應用前景。
產品名稱：

Mouse anti-Homo sapiens (Human) KRT8 Monoclonal antibody
Uniprot No.：

P05787
基因名：

KRT8
別名：

CARD2 antibody; CK 8 antibody; CK-8 antibody; CK8 antibody; CYK8 antibody; CYKER antibody; Cytokeratin endo A antibody; Cytokeratin-8 antibody; DreK8 antibody; EndoA antibody; K2C8 antibody; K2C8_HUMAN antibody; K8 antibody; Keratin 8 antibody; Keratin type II cytoskeletal 8 antibody; Keratin; type II cytoskeletal 8 antibody; Keratin-8 antibody; KO antibody; Krt 2.8 antibody; KRT8 antibody; MGC118110 antibody; MGC174782 antibody; MGC53564 antibody; MGC85764 antibody; sb:cb186 antibody; Type-II keratin Kb8 antibody
宿主：

Mouse
反應種屬：

Human
免疫原：

Synthesized peptide derived from human Cytokeratin 8
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2a, Kappa
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

10E1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
基因功能參考文獻：
1. KRT8 mRNA and protein are upregulated in anaplastic thyroid carcinoma. KRT8 protein binds to annexin A2. PMID: 29443941
2. K8/K18 variants are overrepresented in Chinese NAFLD patients and might accelerate liver fat storage through insulin resistance. PMID: 28652657
3. regulated enhancement of autophagy with concurrent increased expression of KRT8 and the inhibition of KRT8 phosphorylation serve to inhibit oxidative stress-induced epithelial-mesenchymal transitionof retinal pigment epithelium cells. PMID: 28045574
4. Histopathological differences between Magnetic resonance-guided high-intensity focused ultrasound (MR-HIFU)-ablated tissue and radiofrequency (RF)-ablated tissue were demonstrated. CK-8 was more reliable for cell viability assessment than H&E when used directly after ablation PMID: 26732321
5. Loss of activating transcription factor 3 (ATF3) in knockout mice promotes the emergence of keratins CK5+CK8+ epithelial cells. PMID: 26522727
6. notochord-specific marker during early intervertebral disc development PMID: 26910849
7. Data show that the filament elongation of both desmin and keratin K8/K18 proceeds very similar to that of vimentin. PMID: 27304995
8. New insights into interactions between the nucleotide-binding domain of CFTR and keratin 8. PMID: 27870250
9. We present results of a multicentre study measuring UBC((R)) Rapid Test(test that detects fragments of cytokeratins 8 and 18 in urine) in bladder cancer patients and healthy controls with focus on carcinoma in situ (CIS) and high-grade bladder cancer. PMID: 28468590
10. Data show that after siRNA transfection, TGF-beta1 protein level was decreased, and CK18 proteins was decreased, while CK8 proteins was increased, and TERT protein expression was slightly increased at 96 h. PMID: 26462837
11. The interplay between Solo protein and keratin 8/keratin 18 filaments plays a crucial role in tensile force-induced RhoA activation and consequent actin cytoskeletal reinforcement in endothelial cells. PMID: 26823019
12. K8/18 filaments provide resistance to apoptosis in granulosa cell tumor cells by impairing FAS expression. PMID: 26911253
13. These metastatic tumors revealed no detectable expression of CK8/18, E-cadherin, VCAM-1, and ICAM-1 PMID: 26847082
14. Data show that loss of epithelial membrane protein 2 (EMP2) is involved in sphingosylphosphorylcholine (SPC)-induced phosphorylation of keratin 8 (K8) via ubiquitination of protein phosphatase 2 (PP2A) through alpha4 phosphoprotein by caveolin-1 (cav-1). PMID: 26876307
15. The reactivity of rheumatoid arthritis (RA) serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus patients, confirming K8 as a novel autoantigen of RA. PMID: 26277397
16. Variants in highly conserved residues of K8 Lys393Arg (ezetimibe/simvastatin-related) was found in patients with fatal Drug-induced liver injury. PMID: 26286715
17. Keratins 8 and 18 are type II acute-phase responsive genes overexpressed in human liver disease. PMID: 24930437
18. In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed PMID: 26280121
19. Human KRT8 variants promote acetaminophen induced hepatotoxicity in mouse models. PMID: 25963979
20. Syndecan-1 is involved in morphogenesis of the developing tooth crown and cervical loops, andntogether with CK8 and vimentin in differentiation of preameloblasts and preodontoblasts. PMID: 25120060
21. K8 immunostaining of tissue from the lymph node metastasis allows defining a sub-group of lymph node-positive breast cancer patients with a highly unfavourable outcome PMID: 25724181
22. This is the first time intestinal cells have been suggested also functionally impaired by K8/K18 mutations. PMID: 24915158
23. Study indicates that Piwil2 plays a role in Fas-mediated apoptosis and can affect p53 phosphorylation in tumor cells, revealing a novel mechanism of Piwil2 in apoptosis, and supports that Piwil2 plays an active role in tumorigenesis. PMID: 25113562
24. An increased expression of cytokeratin 8 and weak of calponin in tunica media of saphenous vein grafts might be useful markers of unfavorable long-term prognosis in CABG patients. PMID: 24338534
25. These data support the idea that CK8 is an important receptor for Pet on epithelial cells for starting its cytotoxic effects. PMID: 24327340
26. parkin may inhibit cell growth, at least in part, by decreasing RPSA expression and inducing phosphorylation of cytokeratin 8/18 PMID: 23990477
27. These findings, along with the emerging role of cell surface CK8 in cancer metastasis, may imply a dual strategy for tumor cell survival in the host. PMID: 24183726
28. CK8 up-regulation might have a functional role in HCV infection and pathogenesis, and its ectopic expression induces apoptosis of SMMC7721 cells. PMID: 24115814
29. this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence PMID: 23801880
30. Compressive loads resulted in phosphorylation and disassembly of CK8 in nucleus pulposus cells. PMID: 24166186
31. A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization. PMID: 24003221
32. These results indicate the role of K8/18 in modulating invasion in breast cancer. PMID: 23341946
33. Report keratin 8 expression in breast papillomas and papillary carcinoma. PMID: 23327593
34. K8/18 expression can influence the phenotype of epithelial cancer cells at a transcriptional level and supports the hypothesis that keratins play an active role in cancer progression. PMID: 23449973
35. Keratin variants are expressed in a low percentage of patients with alcoholic cirrhosis and do not influence hepatocellular carcinoma development. PMID: 23078008
36. There is aberrant expression of CK8 in dysplasia, oral submucous fibrosis, and oral squamous cell carcinoma, suggesting CK8 as a surrogate marker of malignant transformation. PMID: 22677743
37. K8 acetylation at Lys-207, a highly conserved residue among type II keratins, is up-regulated upon hyperglycemia and down-regulated by SIRT2. PMID: 23358244
38. A key K8/K18 regulation of glucose metabolism in normal and cancerous hepatic cells through differential modulations of mitochondrial hexokinase status and insulin-mediated signaling. PMID: 23164509
39. Fibroblasts prepared from different types of malignant tumors stimulate expression of luminal marker keratin 8. PMID: 22270320
40. Keratin filaments were assembled from recombinant keratin proteins 8 (K8) and 18 (K18) in the presence of divalent ions (Mg(2+)). PMID: 22853896
41. Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells. PMID: 22344252
42. Results show K8 is present on the cell surface of transformed prostate cancer cell lines, and K8 increased the activity of plasmin approximately fivefold over a 48-h period. K8 also enhanced the plasmin-mediated proteolysis of vitronectin. PMID: 22354665
43. In patients with hemochromatosis, intronic but not exonic K8/K18 variants associate with liver fibrosis development. PMID: 22412904
44. We have found the moderate positive correlation between proliferation and a keratin 8 expression in neoplastic epithelial cells. PMID: 22379894
45. previously undescribed K8 variants associate with cryptogenic hepatitis in a German cohort of patients, possibly predisposing carriers to the development of liver disease. PMID: 22419260
46. Loss of keratin 8 phosphorylation leads to increased oral squamous cell carcinoma progression PMID: 22114688
47. Suggest that CK8 is unsuitable for early diagnosis of tongue squamous cell carcinoma. PMID: 21705376
48. the mild KRT8 allele is associated with CFTR mediated residual chloride secretion among F508del-CFTR homozygotes PMID: 21548936
49. The study demonstrates the strong expression of CK8 in limbal epithelial basal cells, which is maintained during the differentiation and migration of the limbal cells toward the central corneal epithelium. PMID: 20926822
50. Clumping factor B is capable of binding cytokeratin 8 during infection. PMID: 20817646
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相關疾病：

Cirrhosis (CIRRH)
亞細胞定位：

Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
蛋白家族：

Intermediate filament family
組織特異性：

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
數據庫鏈接：

HGNC: 6446

OMIM: 148060

KEGG: hsa:3856

STRING: 9606.ENSP00000293308

UniGene: Hs.533782

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