FLOT1 Monoclonal Antibody

說明書

中文名稱：

FLOT1鼠單克隆抗體
貨號：

CSB-MA008727A0m
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, 3T3 whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 48, 43 kDa
Observed band size: 48 KDa
Exposure time：5min

IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human cervical cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA008727A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Overlay Peak curve showing Hela cells stained with CSB-MA008727A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

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靶點詳情

產品詳情

產品描述：

FLOT1單克隆抗體（CUSABIO貨號：CSB-MA008727A0m）是針對脂筏標記蛋白FLOT1研發的高特異性科研工具，該靶點在維持細胞膜微結構域穩定性、跨膜信號轉導及囊泡運輸過程中發揮關鍵作用，其異常表達與腫瘤微環境調控及神經退行性疾病進程密切相關。本產品經多平臺驗證，在Western Blot實驗中可清晰識別約47kDa的目標條帶，在免疫組化、免疫熒光實驗中展現優異定位性能，流式細胞分析中能有效檢測內源性FLOT1表達水平，同時兼容ELISA檢測體系，滿足多維度科研需求。抗體經嚴格的交叉反應驗證，對人源及小鼠樣本均表現出高度反應性，適用于細胞生物學研究、分子互作分析及疾病機制探索等科研場景。在腫瘤生物學領域可用于研究脂筏介導的受體信號通路，在神經科學方向有助于解析阿爾茨海默病等疾病中膜蛋白異常聚集機制，為研究細胞膜動態調控、腫瘤轉移標志物篩選及神經退行性疾病模型構建提供可靠工具。該抗體具有高批次穩定性和低背景特性，適用于冰凍切片、細胞爬片及多種裂解液處理樣本，是探索FLOT1功能及調控機制的理想選擇。
產品名稱：

Mouse anti-Homo sapiens (Human) FLOT1 Monoclonal antibody
Uniprot No.：

O75955
基因名：

FLOT1
別名：

FLOT 1 antibody; FLOT1 antibody; FLOT1_HUMAN antibody; Flotillin-1 antibody; Flotillin1 antibody; Integral membrane component of caveolae antibody; Reggie 2 antibody
宿主：

Mouse
反應種屬：

Human, Mouse
免疫原：

Recombinant FLOT1 protein (149-427AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2a
純化方式：

>95%, Protein A purified
克隆號：

4C8B6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	WB：1:1000-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
基因功能參考文獻：
1. flotillin-1 is a tumorigenic protein that plays an important role in promoting the proliferation and tumorigenicity of LUAD. PMID: 28825855
2. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
3. Autoantibodies against flotillin-1/2 heterocomplex are present in 1-2% of patients with bona fide multiple sclerosis. PMID: 28645295
4. Furthermore, we identify flotillin-1 (FLOT1) and histone H1 as downstream factors for cytoplasmic and nuclear pathway of S100A11, which are required for LASP1-S100A11 axis-mediated epithelial-mesenchymal transition and colorectal cancer progression. PMID: 27181092
5. FLOT1 is associated with aggressive characteristics of HCC PMID: 23840303
6. This study presents the endocytic pathways of internalization for muscarinic type 3 receptor and flotillin-1/2 in salivary gland epithelial cells. knockdown of flot-1 or -2 by flotillin-specific siRNA prevented internalization and reduced the endocytic efficiency of muscarinic type 3 receptor. PMID: 27221048
7. Data show that overexpression of 4.1N protein decreased expression of flotillin-1, decreased activation of beta-catenin/Wnt pathway in non-small-cell lung cancer (NSCLC) cells. PMID: 27448302
8. Upregulation of flotillin-1 promotes invasion and metastasis by activating TGF-beta signaling in nasopharyngeal carcinoma. PMID: 26646322
9. Flotillin-1 protein is upregulated in human endometrial cancer and localization shifts from epithelial to stromal with increasing tumor grade PMID: 26682635
10. Expression of flotillin-1 was associated with H-Ras in breast cancer, especially in TNBC (p < 0.001). PMID: 26413934
11. findings revealed the expression of flotillin 1 in gastric cancer samples is significantly higher than that of noncancer/normal tissues; findings suggest that flotillin 1 may be a novel biomarker for gastric cancer PMID: 25948494
12. miR-506 expression was down-regulated in clear cell renal cell carcinoma (ccRCC) and correlated with advanced histologic grade, clinical stage, tumor stage, positive lymph node metastasis, and distant metastasis. FLOT1, a potential target gene of miR-506, was inversely correlated with miR-506 expression in ccRCC tissues. PMID: 25793370
13. polycystins are necessary for assembly of a novel flotillin-containing ciliary signaling complex and provide a molecular rationale for the common renal pathologies caused by OFD1 and polycystin mutations. PMID: 25180832
14. Findings suggest that FLOT1 plays an important role in the proliferation and recurrence of transitional cell carcinoma. PMID: 24890092
15. Downregulation of microRNA-182-5p contributes to renal cell carcinoma proliferation via activating the AKT/FOXO3a signaling pathway with FLOT1 being a target. PMID: 24886554
16. overexpression of FLOT1 can be found in tongue squamous cell cancer patients with higher pathological stage, T classification, N classification or recurrence PMID: 24695539
17. Flotillin-1 mRNA expression is up-regulated in non-small cell lung cancer patients. PMID: 24533441
18. a critical role of flotillin-1 in ROS production mediated by c-Src PMID: 24983503
19. Flotillin-1 regulates oncogenic signaling in neuroblastoma cells by regulating ALK membrane association. PMID: 24830726
20. The FLOT1 is abundantly expressed in term villous placental CTs and endothelial cells, and in comparison, expression of these proteins in the ST is reduced. PMID: 23064789
21. Depletion of either flotillin-1 or flotillin-2 resulted in downregulation of ErbB3 and a selective reduction of ErbB2-ErbB3 receptor complexes. PMID: 24747692
22. study demonstrated that miR-124 might be a tumor suppressor in breast cancer via the regulation of FLOT1 PMID: 24330780
23. We have identified flotillin 1 and 2 as new partners of the cadherin complexes PMID: 24046456
24. These data here provide direct evidences for the molecular interaction and endocytosis of PrP(C) with Flot-1 in the presence of copper ions. PMID: 23625312
25. Patients with higher FLOT1 expression had shorter overall survival time, whereas those with lower FLOT1 expression had longer survival time PMID: 24277378
26. Human group V secretory phospholipase A2 is associated with lipid rafts and internalized in a flotillindependent pathway. PMID: 24042857
27. EGFR overexpression and increase in signaling correlates with flotillin-1 expresion. PMID: 24304721
28. Report role for flotillin 1/2 in interactions of lung epithelial cells with silica nanoparticles. PMID: 22669515
29. we describe how flotillin-1 and -2 contribute to the stabilization of ErbB2 at the cell surface in breast cancer tissue PMID: 22869152
30. Flotillin-1 plays an important role in Oral squamous cell carcinoma (OSCC) development. PMID: 23581411
31. Flotillin-1 interacted with PrPc and promote the endocytosis of PrPc. PMID: 23627023
32. results support predominant formation of flotillin-1 and -2 hetero-oligomers in resting and chemokine-stimulated human T-cells which may importantly contribute to structuring of the uropod. PMID: 23012365
33. a novel signaling network containing FRS2, CAP and flotillin-1 PMID: 22235335
34. receptor-tyrosine kinases may also rely on flotillin-1 upon activation, thus suggesting a general role for flotillin-1 as a novel factor in receptor-tyrosine kinase/MAP kinase signaling. PMID: 22232557
35. Chlamydia pneumoniae intracellular growth was attenuated in the flotillin-1-silenced cells. PMID: 22215737
36. FLOT1 plays an important role in promoting proliferation and tumorigenesis of human breast cancer and may represent a novel prognostic biomarker and therapeutic target for the disease. PMID: 21447726
37. Flot1-enriched membrane microdomains are required for protein kinase C-regulated internalization of the DA transporter (DAT) and the glial glutamate transporter EAAT2. PMID: 21399631
38. flotillins have a role in NPC1L1-mediated cholesterol uptake and NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains are involved in the mechanism for efficient cholesterol absorption PMID: 21187433
39. the cellular pathways used by transportan and transportan 10 (TP10) for protein transduction PMID: 19348413
40. Data show that the BCG phagosome is relatively depleted in LAMP-2, NPC1, flotillin-1, vATPase, and syntaxin 3. PMID: 19815536
41. In hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization. PMID: 20027317
42. Flotillin-1, a marker of rafts, accumulates in lysosomes of neurons in Alzheimer's disease PMID: 14708344
43. ATP-binding cassette, sub-family A (ABC1), member 1 forms a complex with syntaxin 13 and flotillin-1, residing at the plasma membrane and in phagosomes PMID: 15469992
44. It is significant that there is an increased gene expression of flotillin-1 in the Parkinson substantia nigra/ventral tegmental area compared to controls. PMID: 15545008
45. Recombinant flotillin-1 was purified and characterized in in large quantity and high purity. PMID: 15939299
46. Myocilins with mutations such as G364V, K423E, and Y437H on the domain failed to interact with flotillin-1. PMID: 16198165
47. Flotillin-1 small interfering RNA (siRNA) inhibited both clathrin-independent uptake of cholera toxin and endocytosis of a GPI-linked protein. We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells. PMID: 16341206
48. flotillin-1 may recruit amyloid beta (A4) precursor protein to lipid rafts and therefore participate in the localization and processing of APP PMID: 16480949
49. The results showed that flotillin-1 can interact with arginase1, and hence arginase activity was up-regulated by 26.8%. PMID: 17113085
50. These lines of evidence suggested that a Gq-coupled receptor activates specifically p38 MAPK through lipid rafts and Src kinase activation, in which flotillins positively modulate the Gq signaling. PMID: 17307333
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亞細胞定位：

Cell membrane; Peripheral membrane protein. Endosome. Membrane, caveola; Peripheral membrane protein. Melanosome. Membrane raft.
蛋白家族：

Band 7/mec-2 family, Flotillin subfamily
數據庫鏈接：

HGNC: 3757

OMIM: 606998

KEGG: hsa:10211

STRING: 9606.ENSP00000365569

UniGene: Hs.179986

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