Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2)
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中文名稱:人ME2重組蛋白
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品名簡稱:Recombinant Human ME2 protein (Active)
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貨號:CSB-AP000101HU
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說明書:
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規格:¥852
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圖片:
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其他:
產品詳情
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純度:>95% as determined by SDS-PAGE.
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內毒素:Less than 1.0 EU/μg as determined by LAL method.
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生物活性:Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
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基因名:
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Uniprot No.:
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別名:Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD(+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD(+) dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase
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種屬:Homo sapiens (Human)
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蛋白長度:Full Length of Mature Protein
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來源:E.Coli
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分子量:64.4 kDa
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表達區域:19-584aa
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氨基酸序列MLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVITE+HHHHHH
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蛋白標簽:C-terminal 6xHis-tagged
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產品提供形式:Liquid or Lyophilized powder
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緩沖液:0.2μm filtered PBS, pH 7.4. ,lyophilized
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:5-10 business days
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Datasheet & COA:Please contact us to get it.
相關產品
靶點詳情
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基因功能參考文獻:
- ME2 was involved in glioblastoma multiforme growth, invasion, migration, Reactive oxygen species and ATP production. PMID: 27166188
- deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer PMID: 28099419
- Data indicate that malic enzyme 2 knockdown impacts phosphatidylinositol 3-kinases/proto-oncogene protein akt (PI3K/AKT) signaling. PMID: 24957098
- ME2 might be an important factor in melanoma progression and a novel biomarker of invasion. PMID: 25202825
- Three SNP alleles in BRD2, Cx-36, and ME2 and microdeletions in 15q13.3, 15q11.2, and 16p13.11 also contribute risk to juvenile myclonic epilepsy. PMID: 23756480
- Depletion of malic enzyme 2 induced erythroid differentiation in human erythroleukemia cells. PMID: 20824065
- An ME2-centered nine-SNP haplotype, when present homozygously, increases the risk for IGE (odds ratio 6.1; 95% confidence interval 2.9-12.7) compared with any other genotype PMID: 15532013
- Site directed mutagenesis was used to detrmine which amino acids in the active site of human mitochondrial NAD+-dependent malic enzyme are necessary for the inhibitory effects of ATP. PMID: 16171388
- These findings provide a molecular rationale for the role of hS14 in TR-dependent transcriptional activation of the expression of specific genes. PMID: 17418816
- Schizophrenic subjects are identified with mitochondrial genes involved in oxidative metabolism as showing consistently decreased expression, including ME2. PMID: 17638511
- Single nucleotide polymorphism in ME2 gene is associated with acute lymphoblastic leukemia. PMID: 19066393
- ME2 activity in Islets of Langerhans cells was measured by a spectrophotometric enzyme assay by utilizing the distinct kinetic properties. PMID: 19691144
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亞細胞定位:Mitochondrion matrix.
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蛋白家族:Malic enzymes family
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