Sheep Interleukin 12,IL-12 ELISA Kit

Sheep Interleukin 12,IL-12 ELISA Kit

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中文名稱：

綿羊白介素12(IL-12)酶聯(lián)免疫試劑盒
貨號：

CSB-E14017Sh
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

綿羊白介素12(IL-12)酶聯(lián)免疫試劑盒（CSB-E14017Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12含量。IL12是重要的細(xì)胞因子，在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。它能促進(jìn)T細(xì)胞和NK細(xì)胞的活化和增殖，增強機(jī)體免疫應(yīng)答。研究聚焦其信號傳導(dǎo)機(jī)制、與疾病關(guān)聯(lián)。深入了解IL12機(jī)制，有望為免疫相關(guān)疾病如腫瘤、自身免疫病治療帶來新策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產(chǎn)品適用于綿羊免疫相關(guān)疾病的機(jī)制研究，如感染性疾病模型中細(xì)胞免疫應(yīng)答強度的動態(tài)監(jiān)測、疫苗效力評估實驗中的Th1型免疫激活分析，或炎癥性疾病研究中的細(xì)胞因子網(wǎng)絡(luò)調(diào)控解析，為獸醫(yī)科學(xué)及比較醫(yī)學(xué)研究提供精準(zhǔn)的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

IL-12
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	87-99
1:8	Average %	91
	Range %	88-96

回收率：

The recovery of sheep IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-103
EDTA plasma (n=4)	90	86-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.599	2.780	2.690	2.596
1000	2.177	2.261	2.219	2.125
500	1.621	1.730	1.676	1.582
250	1.113	1.056	1.085	0.991
125	0.756	0.782	0.769	0.675
62.5	0.399	0.411	0.405	0.311
31.25	0.280	0.297	0.289	0.195
0	0.097	0.090	0.094

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Clinical significance of thymidine kinase in Egyptian children with acute lymphoblastic leukemia Adel A Hagag et al,MINI SYMPOSIUM: PEDIATRIC ONCOLOGY: ORIGINAL ARTICLE,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.