Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠髓樣細胞觸發性受體2(TREM2)酶聯免疫試劑盒（CSB-EL024405RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TREM2含量。TREM2是一種跨膜受體，主要表達于小膠質細胞，其作用機制涉及感知組織損傷、介導免疫重塑和調控小膠質細胞功能。研究發現，TREM2與多種配體結合后，可激活下游信號通路，影響小膠質細胞增殖、吞噬和炎癥反應，從而參與多種疾病如阿爾茨海默病、代謝綜合征和腫瘤的病理進程。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產品適用于神經免疫機制研究、小膠質細胞功能探索、阿爾茨海默病或代謝相關疾病的動物模型分析等科研場景，為揭示TREM2在病理生理過程中的作用提供可靠工具，實驗結果需結合其他研究手段進行綜合分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on monocytes 2|triggering receptor expressed on myeloid cells 2a
縮寫：

TREM2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	106
	Range %	101-115
1:4	Average %	88
	Range %	84-92
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat TREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.