Rat thrombin-antithrombin complex,TAT ELISA Kit

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中文名稱：

大鼠凝血酶抗凝血酶復(fù)合物(TAT)酶聯(lián)免疫試劑盒
貨號：

CSB-E08432r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠凝血酶抗凝血酶復(fù)合物(TAT)酶聯(lián)免疫試劑盒（CSB-E08432r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的TAT含量。大鼠凝血酶抗凝血酶復(fù)合物（TAT）是凝血酶與抗凝血酶結(jié)合形成的復(fù)合物，反映凝血酶生成和活性，用于評估抗凝藥物效果、研究血栓形成機(jī)制及血液疾病診斷。通過ELISA技術(shù)檢測TAT水平，可監(jiān)測機(jī)體凝血狀態(tài)和血栓前狀態(tài)。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研場景中動態(tài)監(jiān)測凝血激活狀態(tài)，如在心血管疾病模型、血栓性疾病動物實驗或藥物干預(yù)研究中分析凝血與抗凝血平衡，也可用于體外細(xì)胞實驗中探究凝血相關(guān)信號通路或藥物對凝血級聯(lián)反應(yīng)的影響。其寬泛的樣本兼容性支持從基礎(chǔ)研究到藥物開發(fā)的多層次實驗需求，為凝血功能相關(guān)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

TAT
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	97
	Range %	91-105
1:10	Average %	103
	Range %	97-109
1:20	Average %	95
	Range %	90-98
1:40	Average %	96
	Range %	90-102

回收率：

The recovery of rat TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
EDTA plasma (n=4)	94	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.504	2.385	2.445	2.324
1000	1.712	1.633	1.673	1.552
500	1.010	1.001	1.006	0.885
250	0.606	0.625	0.616	0.495
125	0.387	0.396	0.392	0.271
62.5	0.289	0.295	0.292	0.171
31.25	0.193	0.196	0.195	0.074
0	0.120	0.121	0.121