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中文名稱：大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯免疫試劑盒
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貨號：CSB-E08491r
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯免疫試劑盒（CSB-E08491r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TRACP-5b含量。TRACP-5b是一種靶點。它與骨代謝緊密相關，在破骨細胞中高度表達。研究機制方面，主要聚焦于其在破骨細胞活化、骨吸收過程中的作用。檢測其水平變化，有助于評估骨代謝狀態和相關疾病如骨質疏松等的發病機制。試劑盒檢測范圍為0.312 mIU/mL-20 mIU/mL，在骨質疏松機制研究、骨骼疾病模型評估及骨代謝相關藥物開發等領域具有重要科研價值；適用于體外定量分析骨代謝相關動物模型的生物標志物表達水平，為骨生物學研究、藥物干預效果評價及病理機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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縮寫：TRACP-5b
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.312 mIU/mL-20 mIU/mL
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靈敏度：0.078 mIU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 89-95 1:2 Average % 100 Range % 97-103 1:4 Average % 93 Range % 88-97 1:8 Average % 98 Range % 93-102 -
回收率：
The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-96 EDTA plasma (n=4) 95 91-97 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mIU/ml OD1 OD2 Average Corrected 20 2.076 1.988 2.032 1.887 10 1.292 1.196 1.244 1.099 5 0.804 0.820 0.812 0.667 2.5 0.511 0.521 0.516 0.371 1.25 0.324 0.335 0.330 0.185 0.625 0.282 0.278 0.280 0.135 0.312 0.223 0.212 0.218 0.073 0 0.146 0.144 0.145 -
數據處理：
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貨期：3-5 working days
引用文獻
- Unveiling the Potential Effect of Nattokinase Against Radiation-Induced Acute Lung Injury in Rats NM Elbakary, RE Maarouf, AA Hassan,Natural Product Communications,2025
- An aqueous extract of Prunella vulgaris L. ameliorates cadmium-induced bone loss by promoting osteogenic differentiation in female rats L Mo,Food and chemical toxicology,2023
- Radiotherapy-induced bone deterioration is exacerbated in diabetic rats treated with streptozotocin M Jiang,The Brazilian Journal of Medical and Biological Research,2021
- Tianchang Capsule prevents ovariectomy induced osteoporosis in rats H CHEN,Food Science and Technology,2021
- Irisin Has a Protective Role against Osteoporosis in Ovariectomized Rats EN Morgan,BioMed Research International,2021
- Effect of Qing'e Decoction on Leptin/Leptin Receptor and Bone Metabolism in Naturally Aging Rats P Sun,Evidence-Based Complementary and Alternative Medicine,2020
- Differential skeletal response in adult and aged rats to independent and combinatorial stimulation with pulsed electromagnetic fields and mechanical vibration Cai J,FASEB J,2020
- Metformin alleviates hyperglycemia-induced apoptosis and differentiation suppression in osteoblasts through inhibiting the TLR4 signaling pathway Zheng L, et al,Life Sciences,2018
- Bone health consequence of adjuvant anastrozole in monotherapy or associated with biochanin-A in ovariectomized rat model Mohamed AA.et al,Life Sciences,2018
- Boldine isolated from Litsea cubeba inhibits bone resorption by suppressing the osteoclast differentiation in collagen-induced arthritis Zhao H.et al,Int Immunopharmacol.,2017
- Effects of rabeprazole on bone metabolic disorders in a gastrectomized rat model. Yamasaki Y.et al,Biomed Rep.,2016
- Advanced Oxidation Protein Products Accelerate Bone Deterioration in Aged Rats Zeng JH et al,Exp Gerontol,2013
- A role for suppressed bone formation favoring catch-up fat in the pathophysiology of catch-up growth after food restriction Xiangfei Guo et al,European Journal of Nutrition,2011
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