Rat soluble fibrin monomer complex(SFMC)ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠可溶性血纖蛋白單體復(fù)合物(SFMC)酶聯(lián)免疫試劑盒（CSB-E16270r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFMC含量。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于血栓形成機(jī)制研究、心血管疾病動(dòng)物模型評(píng)估、抗凝血藥物篩選等科研領(lǐng)域，特別在深靜脈血栓、彌散性血管內(nèi)凝血（DIC）等病理模型的分子機(jī)制研究中具有重要應(yīng)用價(jià)值。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

SFMC
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 ng/mL-4000 ng/mL
靈敏度：

15.6 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	102
	Range %	97-107
1:400	Average %	91
	Range %	85-97
1:800	Average %	97
	Range %	91-103

回收率：

The recovery of rat SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.