Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

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產品詳情

產品描述：

大鼠正常T細胞表達和分泌因子/趨化因子5(RANTES/CCL5)酶聯免疫試劑盒（CSB-E07398r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的RANTES/CCL5含量。RANTES/CCL5是一種趨化因子，在免疫調節、炎癥反應等方面有重要背景。其研究機制涉及多種細胞，能吸引特定免疫細胞到炎癥部位，參與免疫應答。它與多種疾病如感染、自身免疫病相關，對它的研究有助于深入了解疾病發病機理與治療。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外實驗中對大鼠疾病模型（如關節炎、神經炎癥或病毒感染模型）中RANTES動態表達的定量分析，也可用于藥物研發過程中評價免疫調節劑或抗炎藥物對趨化因子通路的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
縮寫：

RANTES/CCL5
Uniprot No.：

P50231
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-105
1:2	Average %	102
	Range %	97-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	101
	Range %	97-106

回收率：

The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-92
EDTA plasma (n=4)	92	86-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
亞細胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
數據庫鏈接：

STRING: 10116.ENSRNOP00000014865

UniGene: Rn.8019