Rat proteoglycan 4(PRG4) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠蛋白聚糖4(PRG4)酶聯(lián)免疫試劑盒（CSB-E14125r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PRG4含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，科研人員可將其應(yīng)用于大鼠關(guān)節(jié)炎模型構(gòu)建、軟骨退行性病變機(jī)制探索、關(guān)節(jié)液生物標(biāo)志物分析等領(lǐng)域，也可用于評(píng)估運(yùn)動(dòng)損傷或藥物治療對關(guān)節(jié)內(nèi)PRG4表達(dá)的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Plppr2; Lppr2; Prg4; Phospholipid phosphatase-related protein type 2; Lipid phosphate phosphatase-related protein type 2; Plasticity-related gene 4 protein; PRG-4
縮寫：

PRG4
Uniprot No.：

Q6W5G4
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	96
	Range %	92-101
1:4	Average %	102
	Range %	99-103
1:8	Average %	87
	Range %	82-93

回收率：

The recovery of rat PRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	101	98-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.