Rat p53/tumor protein,p53/TP53 ELISA Kit

Rat p53/tumor protein,p53/TP53 ELISA Kit

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中文名稱：

大鼠P53(P53)酶聯(lián)免疫試劑盒
貨號：

CSB-E08336r
規(guī)格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠P53(P53)酶聯(lián)免疫試劑盒（CSB-E08336r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TP53含量。TP53是重要的抑癌基因，也被稱“基因組衛(wèi)士”。其能監(jiān)控細胞基因組完整性，通過調(diào)控下游基因表達來修復受損DNA或誘導細胞凋亡。當發(fā)生突變時，會失去正常功能，與多種腫瘤發(fā)生發(fā)展密切相關，是腫瘤研究的熱門靶點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于體外科研場景中P53蛋白的定量分析，例如腫瘤動物模型研究、基因表達調(diào)控實驗或細胞應激狀態(tài)下蛋白動態(tài)變化的監(jiān)測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TP53
Uniprot No.：

P10361
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-108
1:2	Average %	87
	Range %	82-91
1:4	Average %	90
	Range %	85-94
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.479	2.615	2.547	2.405
400	2.009	1.974	1.992	1.850
200	1.356	1.330	1.343	1.201
100	0.874	0.929	0.902	0.760
50	0.532	0.571	0.552	0.410
25	0.401	0.425	0.413	0.271
12.5	0.297	0.287	0.292	0.150
0	0.140	0.143	0.142

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Thymoquinone controlled obesity and invigorated cognitive and memory performance in rats consuming a high-fat diet via modulating oxidative stress, inflammation and apoptosis MD Mostafa, ME Amer, MA ElKomy, AI Othman,Scientific Reports,2025
Modulation of AMPK by esomeprazole and canagliflozin mitigates methotrexate-induced hepatotoxicity: involvement of MAPK/JNK/ERK, JAK1/STAT3, and PI3K/Akt signaling pathways AM El-Dessouki, ME Kaml, MF El-Yamany,Naunyn-Schmiedeberg's Archives of Pharmacology,2025
The role of melatonin in preventing amiodarone induced rat liver damage D Petrovi?,Canadian journal of physiology and pharmacology,2023
Potential Antitumor Activity of Combined Lycopene and Sorafenib against Solid Ehrlich Carcinoma via Targeting Autophagy and Apoptosis and Suppressing Proliferation TA El,Pharmaceuticals,2024
Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosis RE Mostafa,Life sciences,2023
Neuromodulatory effect of vardenafil on aluminium chloride/D-galactose induced Alzheimer’s disease in rats: emphasis on amyloid-beta, p-tau, PI3K/Akt/p53 pathway, endoplasmic reticulum stress, and cellular senescence HH Awad,Inflammopharmacology,2023
Antioxidant and Hepatoprotective Effects of Fig Fruit Extract with Olive Oil and Date-Palm Fruit Extract on Hepatic Toxicity of Oral Subchronic Exposure to Some Nanoparticles in Wistar Rats AH Fathy,Journal of Food Quality,2023
Biochemical and pathophysiological improvements in rats with thioacetamide induced-hepatocellular carcinoma using aspirin plus vitamin C RR El Sadda,BMC cancer,2023
Biomedical applications of polysaccharide derived from tetrasporophyte tufts of Asparagopsis armata (Falkenbergia rufolanosa): Focus on antioxidant, anti-inflammatory, anti-coagulant and hepato-protective activities A Feki,Algal Research,2023
Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury R Alaaeldin,Cells,2023
Hepatoprotective effect of Spirulina platensis against carbon tetrachloride-induced liver injury in male rats NA Mohamed,J Pharm Pharmacol,2021
Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats Oluwatobi T. Somade,Metabolism Open,2020
Hepatic oxidative stress, up-regulation of pro-inflammatory cytokines, apoptotic and oncogenic markers following 2-methoxyethanol administrations in rats OT Somade,Biochemistry and Biophysics Reports,2020
Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats OT Somade,Metabolism Open,2020
Renoprotective effect of celecoxib against gentamicin-induced nephrotoxicity through suppressing NFκB and caspase-3 signaling pathways in rats Abdelrahman R S, et al,Chemico-Biological Interactions,2019
Venlafaxine mitigates cisplatin-induced nephrotoxicity via down-regulating apoptotic pathway in rats Dalia H.et al,Chemico-Biological Interactions,2018

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功能：

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
相關疾?。?/div>
p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
亞細胞定位：

Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

P53 family
數(shù)據(jù)庫鏈接：

KEGG: rno:24842

STRING: 10116.ENSRNOP00000047840

UniGene: Rn.54443

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.