Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit

Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit

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中文名稱：

大鼠髓過氧化物酶特異性抗中性粒細(xì)胞胞質(zhì)抗體(MPO-ANCA)酶聯(lián)免疫試劑盒
貨號：

CSB-E08675r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠髓過氧化物酶特異性抗中性粒細(xì)胞胞質(zhì)抗體(MPO-ANCA)酶聯(lián)免疫試劑盒（CSB-E08675r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的MPO-ANCA含量。MPO-ANCA（髓過氧化物酶特異性抗中性粒細(xì)胞胞漿抗體）是一種與血管炎和腎小球腎炎密切相關(guān)的自身抗體。其研究機(jī)制主要涉及MPO分子被自身免疫性T/B細(xì)胞識別的抗原表位，以及易感HLA分子、補(bǔ)體活化、凝血和血小板等參與的免疫炎癥反應(yīng)。MPO-ANCA的致病性在動(dòng)物模型和臨床研究中得到證實(shí)，其與疾病活動(dòng)度密切相關(guān)，是治療的關(guān)鍵靶點(diǎn)。試劑盒檢測范圍為0.625 U/mL-40 U/mL，該產(chǎn)品適用于科研場景中血管炎動(dòng)物模型的抗體水平動(dòng)態(tài)監(jiān)測、疾病機(jī)制探索以及藥物干預(yù)效果評價(jià)等研究，為深入解析MPO - ANCA在免疫病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

MPO-ANCA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

0.625 U/mL-40 U/mL
靈敏度：

0.156 U/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-95
1:2	Average %	97
	Range %	91-105
1:4	Average %	96
	Range %	92-100
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	91	84-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
40	2.612	2.752	2.682	2.518
20	2.013	2.076	2.045	1.881
10	1.367	1.304	1.336	1.172
5	0.802	0.837	0.820	0.656
2.5	0.444	0.424	0.434	0.270
1.25	0.351	0.359	0.355	0.191
0.625	0.241	0.225	0.233	0.069
0	0.160	0.167	0.164

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

The immunomodulatory effect of ivermectin on rat vasculitis model TS Sadeq, NH Mohammed, IS Arif,Immunopathologia Persa,2025
Expression and significance of Fractalkine/CX3CL1 in MPO-AAV-associated glomerulonephritis rats J Ma,BMC nephrology,2024
Persistent immune stimulation exacerbates genetically-driven myeloproliferative disorders via stromal remodeling Tripodo C.et al,Cancer Res,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.