Rat malonyl coenzyme A ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠丙二酸單酰輔酶A(malonyl CoA)酶聯免疫試劑盒（CSB-E12893r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的malonyl CoA含量。Malonyl CoA即丙二酸單酰輔酶A，是脂肪酸合成過程中的關鍵中間產物。其在細胞能量代謝、脂肪酸合成及氧化平衡等方面發揮重要作用。研究聚焦于其合成與代謝調控機制，如關鍵酶的作用，以深入了解代謝紊亂疾病發病機制并探索潛在治療靶點。試劑盒檢測范圍為1.25 ng/mL-20 ng/mL，能量代謝調控機制、肥胖及糖尿病模型研究、心血管疾病相關脂代謝異常探索、脂肪酸代謝通路研究、藥物干預效果評估、營養代謝調控實驗本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

malonyl CoA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 ng/mL-20 ng/mL
靈敏度：

0.625 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	92-103
1:200	Average %	102
	Range %	100-104
1:400	Average %	93
	Range %	88-97
1:800	Average %	89
	Range %	84-93

回收率：

The recovery of rat malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.