Rat macrophage inflammatory protein 2,MIP-2 ELISA kit
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中文名稱：大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E07419r
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)酶聯(lián)免疫試劑盒（CSB-E07419r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CXCL2含量。CXCL2是重要靶點(diǎn)。它屬于趨化因子家族，在炎癥反應(yīng)、腫瘤微環(huán)境等中起關(guān)鍵作用。其研究機(jī)制主要圍繞與受體結(jié)合，介導(dǎo)細(xì)胞趨化和激活，影響免疫細(xì)胞招募、腫瘤細(xì)胞遷移與增殖，目前是炎癥性疾病和腫瘤治療研究熱點(diǎn)。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，靈敏度為19.5 pg/mL。適用于科研領(lǐng)域中對(duì)炎癥機(jī)制、藥物干預(yù)效果及疾病動(dòng)物模型的研究，例如通過檢測(cè)組織勻漿中的MIP - 2水平評(píng)估局部炎癥程度，或分析血液樣本中趨化因子動(dòng)態(tài)變化以探索全身性炎癥反應(yīng)的調(diào)控通路。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Cxcl2 ELISA kit; Cinc3 ELISA kit; Mip-2 ELISA kit; Mip2 ELISA kit; Scyb2C-X-C motif chemokine 2 ELISA kit; Cytokine-induced neutrophil chemoattractant 3 ELISA kit; CINC-3 ELISA kit; Macrophage inflammatory protein 2 ELISA kit; MIP2 ELISA kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：78 pg/mL-5000 pg/mL
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靈敏度：19.5 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Immunology
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
88
Range %
80-95
1:2
Average %
90
Range %
85-99
1:4
Average %
98
Range %
94-101
1:8
Average %
93
Range %
86-98
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回收率：
The recovery of rat MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-98
EDTA plasma (n=4)
89
84-97
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
5000
2.899
2.934
2.916
2.778
2500
2.437
2.567
2.502
2.364
1250
1.817
1.828
1.822
1.684
625
1.228
1.229
1.229
1.091
312
0.736
0.721
0.729
0.591
156
0.445
0.453
0.449
0.311
78
0.263
0.254
0.259
0.121
0
0.141
0.135
0.138
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數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Rutin attenuates bleomycin-induced acute lung injury via miR-9-5p mediated NF-κB signaling inhibition: network pharmacology analysis and experimental evidence FS Alatawi, AME Omran, E Rashad,Frontiers in Pharmacology,2025
- Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary W Lu,American journal of respiratory cell and molecular biology,2022
- Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,BIOMED RESEARCH INTERNATIONAL,2020
- Annexin V homodimer protects against ischemia reperfusion-induced acute lung injury in lung transplantation Hashimoto K.et al,J Thorac Cardiovasc Surg.,2016
- Endotoxin-induced lung alveolar cell injury causes brain cell damage Rodr鉚guez-Gonz謾lez R.et al,Exp Biol Med (Maywood).,2015
- Annexin V Homo-dimer Protects against Ischemia Reperfusion-Induced Acute Lung Injury in Lung Transplantation Kohei Hashimoto. et al,The Journal of Thoracic and Cardiovascular Surgery,2015
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靶點(diǎn)詳情
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最新研究進(jìn)展：CXCL2是介導(dǎo)中性粒細(xì)胞、嗜酸性粒細(xì)胞和巨噬細(xì)胞趨化的重要化學(xué)趨化因子。最新研究表明，CXCL2在許多炎癥疾病中起重要作用，如哮喘、炎性腸病、骨關(guān)節(jié)炎等。此外，CXCL2還參與了腫瘤發(fā)生、發(fā)展和轉(zhuǎn)移過程，是潛在的抗腫瘤治療靶點(diǎn)。
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功能：Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
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基因功能參考文獻(xiàn)：
- this study shows elevated expression of CINC-3 at the site of inflammation as well as their significant reflection in the circulation, thereby suggesting their frontline role in carrageenan-induced acute inflammation PMID: 27967265
- rutin is a potential protective agent in spinal cord injury enhancing the neurotrophic effect by inhibiting the expression of MIP-2 and activation of MMP-9, and downregulating the expression of p-Akt. PMID: 26502930
- The rat spiral ligament fibrocytes were found to release CXCL2 in response to nontypeable H. influenzae via activation of c-Jun, leading to the recruitment of polymorphonuclear cells to the cochlea. PMID: 22379036
- CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats. PMID: 20034917
- Results identify FQHPSFI peptide may be used for the modulation of lipopolysaccharide-stimulated MIP-2 production in alveolar macrophagess. PMID: 20186460
- chemokine production by rat alveolar macrophages is inhibited by taurine chloramine PMID: 11716962
- Intratracheal LPS induced a significant increase in MIP-2 in bronchoalveolar lavage (BAL) fluid with no detectable MIP-2 in the plasma. PMID: 11837784
- demonstrated that the beta-glucan component of the Pneumocystis carinii cell wall is able to stimulate alveolar epithelial cells to produce MIP-2 PMID: 11906040
- role for the nitric oxide pathway in the overproduction of pro-inflammatory mediators IL-6 and MIP-2 during GBS-induced lung inflammation. PMID: 12661899
- junctional epithelium cells produced MIP-2 and CINC-2 in response to LPS stimulation, suggesting that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation PMID: 15045510
- macrophage inflammatory protein 2 mediates platelet-activating factor-induced intestinal injury PMID: 15319184
- no significant differences were detected for MIP-2 levels in diabetic rats when compared with non-diabetic rats after hypoxia PMID: 16132691
- Following cecal ligation and puncture Cxcl2 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
- MIP-2/CXCL2 and MCP-1/CCL2 are increased after injury, and neurons appear to be the source of this expression. Chemokine expression was selectively inhibited by dexamethasone. PMID: 19210118
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亞細(xì)胞定位：Secreted.
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蛋白家族：Intercrine alpha (chemokine CxC) family
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組織特異性：At least expressed in the lung and trachea.
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數(shù)據(jù)庫(kù)鏈接：
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