Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit
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中文名稱(chēng)：大鼠巨噬細(xì)胞炎癥蛋白1α(MIP-1α/CCL3)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E07422r
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠巨噬細(xì)胞炎癥蛋白1α(MIP-1α/CCL3)酶聯(lián)免疫試劑盒（CSB-E07422r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CCL3含量。CCL3作為靶點(diǎn)，背景上它是一種趨化因子，在免疫調(diào)節(jié)、炎癥反應(yīng)等生理病理過(guò)程中發(fā)揮重要作用。研究機(jī)制方面，主要聚焦其與受體結(jié)合激活信號(hào)通路，調(diào)節(jié)免疫細(xì)胞遷移和活化，探索其在疾病發(fā)生發(fā)展中的作用及潛在治療價(jià)值。試劑盒檢測(cè)范圍為12.5 pg/mL-800 pg/mL，適用于炎癥機(jī)制研究、免疫調(diào)節(jié)藥物體外實(shí)驗(yàn)驗(yàn)證、疾病模型中的趨化因子動(dòng)態(tài)分析等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit
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縮寫(xiě)：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：12.5 pg/mL-800 pg/mL
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靈敏度：3.12 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Immunology
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 84-98 1:2 Average % 96 Range % 91-104 1:4 Average % 99 Range % 96-105 1:8 Average % 95 Range % 89-99 -
回收率：
The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 104 99-108 EDTA plasma (n=4) 95 91-104 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 800 2.152 2.051 2.102 1.969 400 1.484 1.383 1.434 1.301 200 0.888 0.867 0.878 0.745 100 0.522 0.511 0.517 0.384 50 0.336 0.347 0.342 0.209 25 0.253 0.264 0.259 0.126 12.5 0.195 0.199 0.197 0.064 0 0.132 0.133 0.133 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,BIOMED RESEARCH INTERNATIONAL,2020
- Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain Hypersensitivity in a Rat Model of Inflammatory Pain Zhenzhao Luo, et al,Cellular and Molecular Neurobiology,2019
- Myeloid sirtuin 6 deficiency accelerates experimental rheumatoid arthritis by enhancing macrophage activation and infiltration into synovium Woo SJ, et al,Ebiomedicine,2018
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相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
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基因功能參考文獻(xiàn)：
- The important chemokine macrophage inflammatory protein1alpha increases inflammatory responses and the secretion of inflammatory molecules from Kupffer cells, including tumor necrosis factoralpha, interleukin (IL)1beta and IL6. PMID: 28677732
- The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia. PMID: 26550961
- Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
- The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy. PMID: 25127716
- Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury. PMID: 24589480
- NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways. PMID: 22791363
- Our data suggest that free radicals produced in CA1 neurons contribute to the recruitment and activation of microglia and neurodegeneration through MIP-1alpha expression. PMID: 18272692
- In this study we present data on long term increase in CCL3 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
- Data show significant concentration increases for CXCL1 and MIP-1alpha in the brain after soman-induced seizure onset. PMID: 21535896
- The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. PMID: 16804970
- P2Y6 activation by uridine 5'-diphosphate induces Ccl2 and Ccl3 expression in primary cultured microglia, astrocytes, and brain slices from neonatal and adult rats. PMID: 21317391
- a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2XR signaling that increases MIP-1alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. PMID: 21162127
- These results suggest that MIP-1alpha is directly involved in the neurodegeneration induced in rats by PVC-211 murine leukemia virus by recruiting macrophages/microglia from the periphery into regions of the brain that eventually become diseased. PMID: 21051067
- expression is regulated by nicotine in alveolar macrophages PMID: 12101081
- The constitutive patterns of MIP-1 alpha mRNA and protein expression are demonstrated in rat brain throughout postnatal development within local microenvironments where the postnatal cell migration phenomenon occurs. PMID: 12899200
- Oligodendroglia express the CCL3 receptor CCR1, but not CCR5. CCL3 induced an intracellular rise in Ca(2+), a typical signalling event of G protein-coupled receptors. PMID: 14583384
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亞細(xì)胞定位：Secreted.
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蛋白家族：Intercrine beta (chemokine CC) family
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