Rat leukemia inhibitory factor,LIF ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠白血病抑制因子(LIF)酶聯免疫試劑盒（CSB-E07432r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、支氣管肺泡灌洗液、組織勻漿樣本中的LIF含量。LIF（白血病抑制因子）是一種多效性細胞因子，屬于IL-6家族，參與細胞生長、分化和免疫反應。其研究機制涉及調節細胞增殖、分化和表型，影響骨代謝、神經發育等。LIF通過與其受體結合，激活JAK/STAT和MAPK信號通路，參與多種疾病的發病過程。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于呼吸系統疾病模型研究、干細胞分化調控實驗及炎癥相關機制探索，可為肺纖維化、急性肺損傷等動物模型中的LIF動態監測提供可靠工具，助力科研人員深入解析LIF在組織再生、免疫微環境調節中的生物學功能。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
縮寫：

LIF
Uniprot No.：

P17777
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	93
	Range %	89-97
1:4	Average %	99
	Range %	93-104
1:8	Average %	90
	Range %	86-98

回收率：

The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-92
EDTA plasma (n=4)	95	89-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
亞細胞定位：

Secreted.
蛋白家族：

LIF/OSM family
數據庫鏈接：

STRING: 10116.ENSRNOP00000009313

UniGene: Rn.44379