Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

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中文名稱：

大鼠硫酸乙酰肝素蛋白多糖2(HSPG2)酶聯免疫試劑盒
貨號：

CSB-EL010868RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

大鼠硫酸乙酰肝素蛋白多糖2(HSPG2)酶聯免疫試劑盒（CSB-EL010868RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、支氣管肺泡灌洗液、尿液、組織勻漿樣本中的HSPG2含量。HSPG2 即硫酸乙酰肝素蛋白聚糖 2，是細胞外基質的重要成分。它參與細胞黏附、增殖等過程。研究機制方面，其異常表達與腫瘤生長、侵襲和轉移密切相關，還涉及多種疾病的病理進程，是頗具潛力的疾病診斷與治療靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，特別適用于體外實驗模型中HSPG2表達的動態監測，例如細胞外基質相互作用研究、血管生成機制探索或組織修復模型的建立。其寬泛的樣本兼容性為科研人員提供了靈活選擇實驗樣本的條件，支持從體液到組織水平的系統性研究。為分子生物學、細胞生物學及基礎醫學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

RP11-132G19.2, PLC, PRCAN, SJA, SJS, SJS1, Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia)|endorepellin (domain V region)|heparan sulfate proteoglycan of basement membrane|perlecan proteogly
縮寫：

HSPG2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSPG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	83
	Range %	80-86
1:400	Average %	97
	Range %	93-101
1:800	Average %	91
	Range %	85-97
1:1600	Average %	100
	Range %	93-107

回收率：

The recovery of rat HSPG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	104	100-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.766	2.798	2.782	2.680
500	2.277	2.286	2.282	2.180
250	1.593	1.562	1.578	1.476
125	0.996	0.963	0.980	0.878
62.5	0.533	0.542	0.538	0.436
31.2	0.384	0.368	0.376	0.274
15.6	0.271	0.291	0.281	0.179
0	0.101	0.103	0.102

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Ischemic Preconditioning Alleviates Cerebral Ischemia–Reperfusion Injury by Interfering With Glycocalyx YN Zhang,Translational Stroke Research,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.