Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
89
Range %
86-93
1:2
Average %
100
Range %
96-104
1:4
Average %
90
Range %
85-94
1:8
Average %
99
Range %
96-102
回收率:
The recovery of rat ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
91-98
EDTA plasma (n=4)
98
95-102
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Neurophysin 2 specifically binds vasopressin.; Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2).
基因功能參考文獻:
Anxiolytic Lactobacillus is found to increase in abundance in inverse proportion to increasing arginine-vasopressin (Avp) gene dosage [most abundant in AVP-deficient Brattleboro (KO) rats]. This genotype effect of Lactobacillus abundance is not found when female rats are analyzed independently. Therefore, Avp deletion affects microbiota composition in a sexually differentiated manner. PMID: 28759308
in the fetal brain whereby vasopressin activates hippocampal interneurons PMID: 29183979
Studies findings imply that arginine vasopressin has a beneficial role for the treatment of cognitive impairments partly through V1a receptor signaling in cerebral hypoperfusion, which is possibly related to improving synaptic plasticity by promoting N-methyl-d-aspartate receptor subunit 2B and postsynaptic density protein externalization and by enhancing autophagy. PMID: 28934194
Data (including data from studies in transgenic rats) suggest diabetes is associated with increased AVP in plasma and in hypothalamic magnocellular neurosecretory cells; electrophysiological recordings in supraoptic nucleus suggest that gamma-aminobutyric acid (GABA) functions as excitatory neurotransmitter in AVP neurons from diabetic rats, whereas it evokes inhibitory responses in AVP neurons from control rats. PMID: 29212780
Vasopressin innervation of the forebrain social behavior neural network undergoes developmental changes and is highly sexually dimorphic. PMID: 28340511
Study shows that acute amphetamine (AMPH) increased vasopressin (AVP) mRNA expression in the medial amygdala, whereas AMPH-induced sensitisation increased AVP mRNA expression in the bed nucleus of the stria terminalis only in females. PMID: 28235136
data suggest that GLP-1 combined with vasopressin could be involved in the regulation of sodium balance in the hyperosmolar state as a result of NaCl loading; vasopressin regulates the reabsorption of a significant portion of filtered sodium in the distal segment of the nephron and modulates the natriuretic effect of GLP-1 PMID: 26791475
Under long term stressed conditions, the release of AVP and OXT from hypothalamus may vary depending on the content of the stressors. Central AVP appears to act oppositely to OXT by mediating CHeS-induced gastric motor maladaptation. Long term central AVP antagonism might be a pharmacological approach for the treatment of stress-related gastric motility disorders. PMID: 27829122
Aroclor-1254 may interfere with the activation of AVP mRNA transcript levels and protein, causing a central dysfunction of vasopressinergic system. PMID: 28579251
This study deminstrated that Depressive- and anxiety-like behaviors and stress-related neuronal activation in vasopressin-deficient female Brattleboro rats. PMID: 26939727
The present study suggests that the Avp gene plays an important role in social development. PMID: 27066536
The results of this study showed that arginine vasopressin increases in both sexs rats with aneurysmal subarachnoid hemorrhage. PMID: 26923576
Chronic mild stress increased arginine vasopressin mRNA levels in the paraventricular nucleus. PMID: 26578428
More AVP was expressed in the paraventricular nucleus after rotatory stimulus in rats susceptible to motion sickness (MS). In the ventricular nucleus, AVP expression were low. Of 9 AVP SNPs, rs105235842 differed significantly with MS susceptibility. PMID: 26651338
This data provides the connection between CREB3L1, a newly identified transcription factor of AVP expression. PMID: 26503226
Local cardiac and circulating AVP secretion were enhanced by deteriorating cardiac function. AVP may promote ventricular remodeling. PMID: 25823554
Regression of Walker 256 carcinosarcoma in vasopressin-deficient Brattleboro rats is accompanied by a changed laminin pattern. PMID: 25854851
AVP appears to play a role in the regulation of impulsivity exclusively during lactation. PMID: 25117459
Active coping toward predatory stress decreases DNA methylation in AVP promoter. PMID: 25127982
Identification of AZIN1, and hence by inference, polyamines as novel regulators of the expression of the AVP gene. PMID: 25961839
Vasopressin-deficient rats that are deprived of their mothers as newborns do not exhibit stress and anxiety-like behaviors as adults, probably as a consequence of reduced ACTH rise immediately following maternal deprivation. PMID: 25462910
This study demonstrated that enhanced amygdalar AVP may be related to individual vulnerability to stress-induced reinstatement of heroin- seeking. PMID: 25446223
Supraoptic nucleus neurons consist of two physiologically distinct populations preferentially expressing oxytocin or vasopressin. PMID: 25451978
results unveiled a unique and complex form of neurovascular coupling supporting a competitive balance between nitric oxide and activity-dependent dendritic released vasopressin. PMID: 25834057
The effects of vasopressin on maternal aggression develop in conjunction with impulsivity. PMID: 25001964
Resistance training did not induce any change in vasopressin levels, and atrial natriuretic peptide levels did not change significantly. However, VP increased after the resting period of 15 days. PMID: 23551170
UT-A1 localized to the apical membrane in response to vasopressin was phosphorylated at Ser(486) and Ser(499). PMID: 25377918
eNOS inhibition in spontaneously hypertensive rats induces or enhances an inverse reciprocal interaction between hypothalamus and left ventricle involving the renin-angiotensin system and vasopressin PMID: 24627106
This study is the first to demonstrate dual vasopressin/apelin adaptation mediated by inflammatory molecules and neuronal Trpv2, during aging. PMID: 24505289
The endocannabinoid anandamide decreases basal oxytocin and vasopressin secretion from rat neurohypophysis. PMID: 24342802
We identify CREB3L1 as a regulator of AVP transcription in the rat hypothalamus. PMID: 24623760
Over-expression of CRH and vasopressin in the magnocellular paraventricular nucleus represents a specific feature of anxiety/post traumatic stress disorder-like state. PMID: 24317347
Suggest that the P2X7R is expressed in neurohypophysial terminals and has a role in AVP secretion. PMID: 24037803
The positive role of arginine vasopressin (AVP) in the regulation of adrenocorticotropic hormone secretion from the pituitary in animals exposed to heat is confirmed. AVP from the general circulation influences pituitary vasopressin 1b receptor. PMID: 23580725
Arginine vasopressin-containing neurons of the magnocellular hypothalamic nuclei serve as important sources for hippocampal innervation. PMID: 23085097
The Notch1-Hes1 signalling axis suppresses neuroendocrine differentiation and maintains tubular/acinar features in adenocarcinoma and non-neoplastic epithelium in the biliary tree. PMID: 23523151
Interactions between corticosterone and catecholaminergic projections to the hypothalamus therefore make significant contributions to the regulation of Crh and Avp expression. PMID: 22831701
Leukotrienes may modulate arginine vasopressin secretion. PMID: 22960410
GLP-1 directly stimulated the promoter activities and mRNA levels of both CRF and AVP in hypothalamic 4B cells PMID: 22801106
These results that AVP excites warm-sensitive neurons and inhibits cold-sensitive and temperature-insensitive neurons PMID: 22677202
No difference is found in either quality or quantity of oxytocin- and vasopressin-immunoreactive neurons between individual rats of different social and reproductive ranks. PMID: 22266748
These data demonstrate direct relationships between enhanced AVP neuronal activation and a potentiated vasopressin system, and this latter one with high conditioned anxiety in MS male rats PMID: 22522466
Chronic psychological stress disrupted fasted motility in the antrum and colon via central urocortin 3/arginine vasopression pathways. PMID: 22651925
Vasopressin was more important in the regulation of the adrenocorticotropin secretion in the perinatal vasopressin-deficient Brattleboro rats than in adulthood, where its role depended on the nature of the stressor encountered. PMID: 22527858
In the mammalian peripartum period, the activity of both the brain oxytocin and vasopressin system is elevated as part of the physiological adaptations occurring in the mother--REVIEW PMID: 22100184
We review this and other work demonstrating that both vasopressin and oxytocin modulate conspecific social recognition at the level of the olfactory system--review PMID: 21920364
initial findings suggest that oxytocin and vasopressin systems may mediate early social environment-induced alterations in social behavior--REVIEW PMID: 22197269
AVP can act within multiple regions social behavior network to regulate the expression of individual social behaviors--REVIEW PMID: 22079778
During protracted withdrawal from cocaine, enhanced AVP gene expression is associated with persistent elevations of basal hypothalamus pituitary axis activity. PMID: 21677651
AVP secretion of the pituitary may be involved in the sex differences in susceptibility to motion sickness. PMID: 21171363