Rat apolipoprotein H (Apo-H) ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠載脂蛋白H(Apo-H)酶聯免疫試劑盒（CSB-E10073r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOH含量。APOH（載脂蛋白H）是一種參與脂質代謝的關鍵蛋白，在調節凝血和炎癥方面發揮著重要作用。研究表明，APOH與多種疾病有關，包括自身免疫性疾病和心血管疾病。其研究機制主要涉及APOH與帶負電物質（如肝素和磷脂）的相互作用，以及其在防止內在凝血級聯激活中的作用。試劑盒檢測范圍為28 ng/mL-1800 ng/mL，適用于大鼠模型中Apo-H的定量分析，可支持脂代謝研究、心血管疾病機制探索以及免疫相關實驗的開展，特別適用于基礎科研中涉及脂蛋白調控、血栓形成或免疫應答機制的相關課題。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
縮寫：

APOH
Uniprot No.：

P26644
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

28 ng/mL-1800 ng/mL
靈敏度：

7 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	102
	Range %	97-107
1:800	Average %	91
	Range %	85-97
1:1600	Average %	97
	Range %	91-103

回收率：

The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	107	101-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
亞細胞定位：

Secreted.
組織特異性：

Expressed by the liver and secreted in plasma.
數據庫鏈接：

UniGene: Rn.1824