Rat Translationally-controlled tumor protein(TPT1) ELISA kit

Rat Translationally-controlled tumor protein(TPT1) ELISA kit

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中文名稱：

大鼠翻譯調(diào)節(jié)腫瘤蛋白(TPT1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL024134RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

大鼠翻譯調(diào)節(jié)腫瘤蛋白(TPT1)酶聯(lián)免疫試劑盒（CSB-EL024134RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TPT1含量。TPT1是一個(gè)重要靶點(diǎn)。它與細(xì)胞內(nèi)多種生理過(guò)程相關(guān)，在腫瘤發(fā)生、發(fā)展等方面可能發(fā)揮作用。研究機(jī)制主要聚焦于其在細(xì)胞增殖、凋亡調(diào)控通路中的作用，探索其作為腫瘤診斷標(biāo)志物和治療靶點(diǎn)的可能，為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為39 pg/mL-2500 pg/mL，適用于腫瘤生物學(xué)研究中的蛋白質(zhì)表達(dá)分析、藥物干預(yù)模型評(píng)價(jià)以及細(xì)胞應(yīng)激狀態(tài)下TPT1動(dòng)態(tài)監(jiān)測(cè)等科研場(chǎng)景，為探索腫瘤發(fā)生機(jī)制及篩選潛在治療靶點(diǎn)提供精準(zhǔn)的體外檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; Lens epithelial protein ELISA Kit
縮寫(xiě)：

TPT1
Uniprot No.：

P63029
種屬：

Rattus norvegicus (Rat)
樣本類(lèi)型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.75 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	94
	Range %	90-98
1:4	Average %	98
	Range %	94-104
1:8	Average %	95
	Range %	88-99

回收率：

The recovery of rat TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	89-104
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.047	2.134	2.091	1.959
1250	1.384	1.483	1.434	1.302
625	0.854	0.836	0.845	0.713
312.5	0.517	0.524	0.521	0.389
156	0.337	0.346	0.342	0.210
78	0.257	0.248	0.253	0.121
39	0.195	0.201	0.198	0.066
0	0.134	0.129	0.132

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Involved in calcium binding and microtubule stabilization.
基因功能參考文獻(xiàn)：
1. Tumor protein translationally-controlled -induced dopamine release from neuronal PC12 cells was modulated by a Ca(2+)-independent mechanism that involved PLA in the process, suggesting the regulatory role of TCTP in the neuronal functions. PMID: 27783042
2. After acute spinal cord injury, TCTP in astrocytes gradually increased, peaking at day 3 and returning to basal levels by day 14. TCTP knockdown reduced astrocyte migration and proliferation. PMID: 26266488
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

TCTP family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:116646

STRING: 10116.ENSRNOP00000001383

UniGene: Rn.156654

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.