Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

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中文名稱：

大鼠組織因子途徑抑制物(TFPI)酶聯免疫試劑盒
貨號：

CSB-E08261r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠組織因子途徑抑制物(TFPI)酶聯免疫試劑盒（CSB-E08261r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TFPI含量。TFPI即組織因子途徑抑制物，是一種天然存在的抗凝蛋白。它能抑制組織因子（TF）介導的凝血途徑，通過與因子Xa和TF - 因子VIIa復合物結合，發揮抗凝作用。其在調節凝血平衡中起關鍵作用，對其研究有助于開發新型抗凝藥物等。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，為研究凝血機制、心血管疾病模型、藥物干預實驗及炎癥相關研究提供可靠工具，特別適用于評估凝血相關因子調控、病理模型建立及治療性化合物篩選等科研場景，所有檢測結果僅用于科學研究目的。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tfpi ELISA Kit; Tissue factor pathway inhibitor ELISA Kit; TFPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; EPI ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; LACI ELISA Kit
縮寫：

TFPI
Uniprot No.：

Q02445
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	89
	Range %	84-95
1:4	Average %	93
	Range %	89-97
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-107
EDTA plasma (n=4)	92	88-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.596	2.474	2.535	2.424
250	1.978	1.902	1.940	1.829
125	1.172	1.096	1.134	1.023
62.5	0.687	0.702	0.695	0.584
31.2	0.401	0.424	0.413	0.302
15.6	0.282	0.291	0.287	0.176
7.8	0.182	0.175	0.179	0.068
0	0.113	0.108	0.111

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Hemostatic effect of acylated ghrelin in control and sleeve gastrectomy-induced rats: mechanisms of action Morsy MD.et al,ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY,2018
Procoagulant and Fibrinolytic Activity after Polytrauma in Rat Wu X. et al,Am J Physiol Regul Integr Comp Physiol,2015

功能：

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
基因功能參考文獻：
1. TFPI expression is induced in cardiomyocytes, but not in endothelial cells, by IL-1beta. PMID: 11816723
2. Rapid atrial pacing acutely downregulated the gene expression of thrombomodulin and TFPI in rat atrial endocardium, thereby inducing local coagulation imbalance on the internal surface of the atrial cavity PMID: 14610015
3. TFPI levels decreased significantly at 24 hours in burn, septic injury, and burn+septic injury groups, followed by incomplete rebound recovery at 72 hours PMID: 15497025
4. This is the first report showing that direct inhibition of TF-mediated coagulation activation abrogates bleomycin-induced pulmonary fibrosis. PMID: 16338226
5. analysis of tissue factor changes and the effects of tissue factor pathway inhibitor on transient focal cerebral ischemia in rats PMID: 18067952
6. The results demonstrated that overexpression of TFPI gene might induce VSMC apoptosis in vitro through the mitochondrial pathway; meanwhile, IAP-1 expression is decreased. PMID: 18368493
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亞細胞定位：

Secreted.
組織特異性：

Most abundant in heart, lung, kidney, and aortic endothelial cells.
數據庫鏈接：

KEGG: rno:29436

STRING: 10116.ENSRNOP00000006787

UniGene: Rn.15795

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.