Rat Runt-related transcription factor 2(RUNX2) ELISA kit

Rat Runt-related transcription factor 2(RUNX2) ELISA kit

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中文名稱：

大鼠Runt相關轉錄因子2(RUNX2)酶聯免疫試劑盒
貨號：

CSB-EL020594RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

大鼠Runt相關轉錄因子2(RUNX2)酶聯免疫試劑盒（CSB-EL020594RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的RUNX2含量。RUNX2是重要的轉錄因子，在骨骼發育和骨代謝中起關鍵作用。其能調控成骨細胞分化和軟骨細胞成熟。研究機制主要聚焦于它與多種信號通路相互作用，如BMP、Wnt等，進而對骨形成相關基因表達進行調節。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于體外科研實驗中探索骨骼發育機制、骨代謝調控、骨質疏松模型評估、骨再生修復研究以及干細胞向成骨細胞分化的動態監測等領域。為骨生物學、骨組織工程及骨疾病機制研究提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit
縮寫：

RUNX2
Uniprot No.：

Q9Z2J9
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-103
1:2	Average %	102
	Range %	94-107
1:4	Average %	88
	Range %	83-91
1:8	Average %	94
	Range %	84-98

回收率：

The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	93-107
EDTA plasma (n=4)	98	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.551	2.452	2.502	2.322
100	2.126	1.996	2.061	1.881
50	1.359	1.341	1.350	1.170
25	0.793	0.818	0.806	0.626
12.5	0.474	0.481	0.478	0.298
6.25	0.395	0.403	0.399	0.219
3.12	0.266	0.257	0.262	0.082
0	0.182	0.178	0.180

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Tofu-Incorporated Hydrogels for Potential Bone Regeneration K Huang,ACS Biomater. Sci. Eng,2020

功能：

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
亞細胞定位：

Nucleus.
數據庫鏈接：

STRING: 10116.ENSRNOP00000057733

UniGene: Rn.83672

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.