Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit
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中文名稱：大鼠前列腺素-H2 D-異構(gòu)酶(PTGDS)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL018969RA
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠前列腺素-H2 D-異構(gòu)酶(PTGDS)酶聯(lián)免疫試劑盒（CSB-EL018969RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、腦脊液（CSF）、組織勻漿樣本中的PTGDS含量。PTGDS即前列腺素D合成酶，在多種生理和病理過(guò)程中發(fā)揮重要作用。其主要催化花生四烯酸代謝生成前列腺素D?。在研究機(jī)制方面，它參與睡眠調(diào)節(jié)、炎癥反應(yīng)等過(guò)程，是神經(jīng)炎癥、疼痛等疾病潛在治療靶點(diǎn)，相關(guān)研究在持續(xù)深入。試劑盒檢測(cè)范圍為9.37 ng/mL-600 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中對(duì)前列腺素代謝通路、神經(jīng)退行性疾病機(jī)制、炎癥相關(guān)信號(hào)傳導(dǎo)等方向的研究，支持多種生物樣本類型的并行檢測(cè)，為體外實(shí)驗(yàn)提供標(biāo)準(zhǔn)化分析工具。實(shí)驗(yàn)操作便捷，配套試劑完整，適用于實(shí)驗(yàn)室常規(guī)檢測(cè)及高通量篩選場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名：Ptgds ELISA Kit; Prostaglandin-H2 D-isomerase ELISA Kit; EC 5.3.99.2 ELISA Kit; Glutathione-independent PGD synthase ELISA Kit; Lipocalin-type prostaglandin-D synthase ELISA Kit; Prostaglandin-D2 synthase ELISA Kit; PGD2 synthase ELISA Kit; PGDS ELISA Kit; PGDS2 ELISA Kit
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縮寫：PTGDS
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
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檢測(cè)范圍：9.37 ng/mL-600 ng/mL
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靈敏度：2.34 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Metabolism
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-100 1:2 Average % 90 Range % 85-94 1:4 Average % 85 Range % 80-89 1:8 Average % 102 Range % 98-107 -
回收率：
The recovery of rat PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 87 82-91 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 600 2.601 2.495 2.548 2.345 300 2.004 1.916 1.960 1.757 150 1.295 1.328 1.312 1.109 75 0.817 0.860 0.839 0.636 37.5 0.479 0.452 0.466 0.263 18.75 0.352 0.339 0.346 0.143 9.37 0.268 0.281 0.275 0.072 0 0.199 0.207 0.203 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Effect of the Biofield Energy Treated Proprietary Test Formulation for Sleep Biomarkers in the Unpredictable Chronic Stress (UCS) Animal Model UCSUCSA Model - jndc-chemistryarticles.info,/,2023
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靶點(diǎn)詳情
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功能：Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR.
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基因功能參考文獻(xiàn)：
- HIF-1alpha-l-PGDS-PPARgamma regulates hypoxia-induced ANP secretion in beating rat atria PMID: 29287795
- Lipocalin-type prostaglandin synthase (L-PGDS) may have a role in sleep and chronic intermittent hypoxia PMID: 28025147
- Anorectic tumor-bearing rats do not display the changes in ventral striatal prostaglandin D synthase expression observed in food-restricted weight-matched animals. PMID: 21334429
- Gene expression is regulated by Hes-1 through E-box and interleukin-1 beta via two NF-kappa B elements in rat leptomeningeal cells. PMID: 12488457
- study showed that L-PGDS was strikingly expressed in the caput epididymidis, while a moderate to weak expression was observed in the corpus and cauda epididymidis PMID: 16730417
- Prostaglandin D synthase and prostacyclin synthase are present and could be regulated by steroids in the rat uterus during pregnancy. Endometrium could be significant source of PGD(2) and PGI(2) at specific times during pregnancy. PMID: 17951541
- These results suggested that the removal of the disulfide bond slightly increased the affinities of L-PGDS for ligand binding by changing the hydrophobic regions. PMID: 18590794
- This cardioprotective effect of dexamethasone was markedly reduced in L-PGDS-deficient mice PMID: 19451694
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亞細(xì)胞定位：Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm, perinuclear region. Secreted.
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蛋白家族：Calycin superfamily, Lipocalin family
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組織特異性：Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. In the eye, it is expressed in the pigmented epithelium of the retina and the nonpigmented epithelium of the iris and ci
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