Rat Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit

說明書下載

產品詳情

產品描述：

大鼠血小板衍生生長因子AB(PDGF-AB)酶聯免疫試劑盒（CSB-E04703r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-AB含量。PDGF - AB是一種重要的靶點。它屬于血小板衍生生長因子家族，在細胞生長、增殖、遷移和血管生成等過程中有關鍵作用。目前研究聚焦于其信號通路機制，探索通過阻斷該靶點來抑制腫瘤生長、纖維化等疾病發展，為相關疾病治療提供新思路。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，特別適用于大鼠模型研究中PDGF - AB的動態監測，例如創傷修復實驗、炎癥反應評估或血管生成相關機制研究，為探索PDGF - AB在組織再生、病理進程中的調控作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PDGF-AB
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	88
	Range %	84-93
1:4	Average %	105
	Range %	101-109
1:8	Average %	100
	Range %	95-106

回收率：

The recovery of rat PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	102	97-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.