Rat Myoglobin,MYO/MB ELISA Kit

Rat Myoglobin,MYO/MB ELISA Kit

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中文名稱：

大鼠肌紅蛋白(MYO/MB)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08049r
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
其他：

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產(chǎn)品描述：

大鼠肌紅蛋白(MYO/MB)酶聯(lián)免疫試劑盒（CSB-E08049r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MB含量。試劑盒檢測(cè)范圍為15.6 ng/mL-1000 ng/mL，運(yùn)動(dòng)生理學(xué)、肌肉疾病模型或心血管研究等領(lǐng)域；實(shí)驗(yàn)室研究肌肉損傷機(jī)制、評(píng)估藥物干預(yù)對(duì)肌肉組織的影響或探索代謝性疾病相關(guān)生物標(biāo)志物等科研需求；肌肉相關(guān)基礎(chǔ)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

Mb ELISA Kit; Myoglobin ELISA Kit
縮寫：

MB
Uniprot No.：

Q9QZ76
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MYO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-100
1:2	Average %	92
	Range %	87-100
1:4	Average %	97
	Range %	90-105
1:8	Average %	87
	Range %	83-96

回收率：

The recovery of rat MYO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-99
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.064	2.069	2.067	1.922
500	1.743	1.754	1.749	1.604
250	1.355	1.363	1.359	1.214
125	0.954	0.962	0.958	0.813
62.5	0.718	0.728	0.723	0.578
31.25	0.498	0.502	0.500	0.355
15.6	0.337	0.343	0.340	0.195
0	0.143	0.146	0.145

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Revolutionizing hyperlipidemia treatment: Nanoencapsulated CoQ10 and selenium combat simvastatin-induced myopathy and insulin resistance in rats HB Abo-zalam,/,2023
A rat model of acute kidney injury caused by multiple subcutaneous injections of Asian giant hornet (Vespa mandarina Smith) venom F Yu,Toxicon,2023
Megalin blockade with cilastatin ameliorates multiple wasp sting-induced acute kidney injury in rats J Han,Toxicon,2022
Magnetic Targeting Improves the Therapeutic Efficacy of Microbubble-Mediated Obstructive Thrombus Sonothrombolysis Chen X, et al,Thrombosis and Haemostasis,2019
Elucidation of the Mechanism of Atorvastatin-Induced Myopathy in a Rat Model El-Ganainy SO.et al,Toxicology.,2016

功能：

Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
基因功能參考文獻(xiàn)：
1. In rhabdomyolytic rats, the level of heme and free iron in cytoplasm and mitochondria of kidney cells is remarkably increased while inhibition of proteolysis results in further elevation of myoglobin content in the renal tissue. PMID: 27329933
2. After reperfusion, there was no significant increase in dialysate myoglobin concentration. MDL-28170 did not affect dialysate myoglobin concentration during occlusion or after reperfusion. PMID: 24256333
3. Amphetamine-induced serum myoglobin release is a potential biomarker and possibly a factor in AMPH-induced toxicity processes. PMID: 23608161
4. bradykinin-induced NO does not interact significantly with cellular Mb to produce an NMR-detectable quantity of metMb in the perfused rat myocardium PMID: 14766675
5. determination of myoglobin concentration and succinate dehydrogenase activity in serial sections indicate that myoglobin can lead to a substantial reduction (18-60%) of the extracellular oxygen tension required to prevent an anoxic core in muscle cells PMID: 15048578
6. Mb-facilitated diffusion has a higher poise in skeletal muscle than in myocardium. PMID: 17038435
7. Moreover, the similar Q10 for the DMb of solution versus cell Mb suggests that any temperature-dependent alteration of the postulated cell matrix does not significantly affect protein mobility. PMID: 17218454
8. The nitrite reductase activity of deoxymyoglobin reduces nitrite approximately 36 times faster than deoxyhemoglobin because of its lower heme redox potential. PMID: 17293481
9. intermittent hypobaric hypoxia decreased myocardial citrate synthase activity and myoglobin and decreased myocardial lactate dehydrogenase activity PMID: 19308875
10. Data suggest that rhabdomyolysis-induced kidney damage involves direct interaction of myoglobin with mitochondria possibly resulting in iron ions release from myoglobin's heme, which promotes the peroxidation of mitochondrial membranes. PMID: 19545623
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蛋白家族：

Globin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:59108

STRING: 10116.ENSRNOP00000006184

UniGene: Rn.40511

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.