Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠巨噬細胞移動抑制因子(MIF)酶聯免疫試劑盒（CSB-E07293r）檢測血清、血漿、組織勻漿樣本中的MIF含量。MIF即巨噬細胞移動抑制因子，是一種多功能細胞因子。在炎癥、腫瘤等疾病中發揮重要作用。其研究機制主要聚焦在它調控細胞增殖、遷移和炎癥反應等方面，通過與受體結合激活下游信號通路，為相關疾病治療提供了潛在靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于科研場景下對大鼠MIF表達水平的動態監測，例如炎癥模型構建、免疫調控機制探索或藥物干預效果評估等研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mif ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Glutathione-binding 13 kDa protein ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
縮寫：

MIF
Uniprot No.：

P30904
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
Range %	90-100
1:10	Average %	98
Range %	92-102
1:20	Average %	94
Range %	89-99
1:40	Average %	92
Range %	82-96

回收率：

The recovery of rat MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	96	92-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
亞細胞定位：

Secreted. Cytoplasm.
蛋白家族：

MIF family
組織特異性：

Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney.
數據庫鏈接：

KEGG: rno:103694877

STRING: 10116.ENSRNOP00000008608

UniGene: Rn.2661