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中文名稱：大鼠L-乳酸脫氫酶(L-LDH)酶聯免疫試劑盒
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貨號：CSB-E11324r
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：大鼠L-乳酸脫氫酶(L-LDH)酶聯免疫試劑盒（CSB-E11324r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDHA含量。LDHA即乳酸脫氫酶A，在糖酵解途徑中發揮關鍵作用，與細胞能量代謝密切相關。異常表達的LDHA與腫瘤的發生、發展、轉移及預后不良相關。目前研究聚焦于抑制LDHA活性或表達來調控腫瘤糖代謝，有望為腫瘤治療提供新策略。試劑盒檢測范圍為39 mU/mL-2500 mU/mL，該產品可應用于科研場景中藥物毒性評估、疾病模型構建、代謝機制研究等領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：39 mU/mL-2500 mU/mL
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靈敏度：9.77 mU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Metabolism
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 80-97 1:2 Average % 92 Range % 88-105 1:4 Average % 97 Range % 92-104 1:8 Average % 94 Range % 86-98 -
回收率：
The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-100 EDTA plasma (n=4) 90 85-95 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mU/ml OD1 OD2 Average Corrected 2500 2.754 2.952 2.853 2.679 1250 2.218 2.376 2.297 2.123 625 1.534 1.584 1.559 1.385 312.5 1.022 1.039 1.031 0.857 156 0.587 0.624 0.606 0.432 78 0.412 0.430 0.421 0.247 39 0.286 0.279 0.283 0.109 0 0.173 0.175 0.174 -
數據處理：
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貨期：3-5 working days
引用文獻
- Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers EA Archibong, JA Beshel, IA Okon,Journal of Pharmacopuncture,2024
- The ameliorative role of germinated fenugreek and barley grains on the structure and function of offspring's tibialis muscle maternally fed on high fat diet FA Hassan Megahed,/,2024
- Improved cardioprotective effect of 3‐nitro‐N‐methyl salicylamide solution after a prolonged preservation time of rat heart S Wang,Clinical and experimental pharmacology & physiology,2023
- Magnetic vagus nerve stimulation alleviates myocardial ischemia-reperfusion injury by the inhibition of pyroptosis through the M2AChR/OGDHL/ROS axis in rats Y Lu,Journal of nanobiotechnology,2023
- The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy J Seok,Antioxidants,2023
- Effects of heat shock protein 90 on complement activation in myocardial ischemia/reperfusion injury after pioglitazone preconditioning D Wang,Advances in clinical and experimental medicine,2023
- Nano-Encapsulation of Doxorubicin Using Pectin: Safety an Activity on Chemotherapy-Induced Cardiotoxicity in Carcinoma Mice N Mokhtar,nanobioletters,2022
- The synergistic mechanism of total saponins and flavonoids in Notoginseng?Safflower pair against myocardial ischemia uncovered by an integrated metabolomics strategy Y Meng,Biomedicine & Pharmacotherapy,2020
- Involvement of HSP90 in ischemic postconditioning-induced cardioprotection by inhibition of the complement system, JNK and inflammation Wang DX,Acta Cir Bras,2020
- Protective effect of tanshinone IIA on H2O2-induced oxidative stress injury in rat cardiomyocytes by activating Nrf2 pathway G Yang,J. Recept. Signal Transduct. Res,2020
- Donor Brain Death Leads to a Worse Ischemia-Reperfusion Injury and Biliary Injury After Liver Transplantation in Rats Chen S,Transplantation Proceedings,2020
- Microarray Analysis For Expression Profiles of lncRNAs and circRNAs in Rat Liver after Brain-Dead Donor Liver Transplantation Sanyang Chen, et al,Biomed Research International,2019
- The protective effect of kaempferol on heart via the regulation of Nrf2, NF-κβ, and PI3K/Akt/GSK-3β signaling pathways in isoproterenol-induced heart failure in diabetic rats Zhang L, et al,Drug development research,2019
- Pretreatment with low-dose gadolinium chloride attenuates myocardial ischemia/reperfusion injury in rats Chen M.et al,Acta Pharmacol Sin.,2016
- Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015
- Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction Nirala BK. et al,Diab Vasc Dis Res,2015
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靶點詳情
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基因功能參考文獻：
- LDHA is a direct target of miR-34a, which binds to the 3'-UTR region of LDHA mRNA in cardiomyocytes. PMID: 28894025
- role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells PMID: 28818664
- After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function. PMID: 27654895
- The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
- aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration PMID: 24835193
- Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. PMID: 24086675
- lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
- M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967
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亞細胞定位：Cytoplasm.
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蛋白家族：LDH/MDH superfamily, LDH family
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數據庫鏈接：
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