Rat Keratinocyte Growth Factor,KGF ELISA Kit

Rat Keratinocyte Growth Factor,KGF ELISA Kit

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文獻引用 (1) 說明書下載

中文名稱：

大鼠角化細胞生長因子(KGF)酶聯(lián)免疫試劑盒
貨號：

CSB-E12905r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

大鼠角化細胞生長因子(KGF)酶聯(lián)免疫試劑盒（CSB-E12905r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的FGF7含量。FGF7即成纖維細胞生長因子 7，屬于FGF家族。它在胚胎發(fā)育、組織修復等過程發(fā)揮重要作用，可促進上皮細胞的增殖、遷移等。其研究機制主要圍繞與受體結合激活下游信號通路，在腫瘤、皮膚疾病等領域是潛在治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于皮膚損傷修復機制、上皮細胞再生調控、藥物干預效果評估等基礎研究領域，為探索KGF在組織穩(wěn)態(tài)維持及疾病模型中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Fgf7 ELISA Kit; Fgf-7 ELISA Kit; KgfFibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit
縮寫：

FGF7
Uniprot No.：

Q02195
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-95
1:2	Average %	95
	Range %	89-101
1:4	Average %	92
	Range %	87-96
1:8	Average %	86
	Range %	80-93

回收率：

The recovery of rat KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	94-105
EDTA plasma (n=4)	95	89-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.730	2.775	2.753	2.621
1000	2.226	2.403	2.315	2.183
500	1.500	1.610	1.555	1.423
250	0.837	0.890	0.864	0.732
125	0.428	0.432	0.430	0.298
62.5	0.221	0.235	0.228	0.096
31.2	0.167	0.158	0.163	0.031
0	0.130	0.133	0.132

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Mesenchymal stem cells overexpressing heme oxygenase-1 ameliorate lipopolysaccharide-induced acute lung injury in rats Chen X, et al,Journal of Cellular Physiology,2018

功能：

Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
基因功能參考文獻：
1. The findings of the present study indicated that KGF, CTGF and TGF-beta have biological roles in progression of CsA- and PNT- induced GO. KGF plays a greater role in CsA- induced GO than in PNT- induced GO. Meanwhile, CTGF and TGF-beta play a role in PNT- induced GO greater than in CsA- induced GO. PMID: 26894526
2. These data demonstrate that administration of MSCs to the inflammation-insulted AT-II cells may ameliorate the impairments through a KGF-dependent PI3K/AKT/mTOR signaling pathway. PMID: 27035760
3. The primary role of both KGF1 and FGF2 appeared to be in the proliferation and migration of keratinocytes PMID: 21136143
4. KGF may have a role in ameliorating radiation-induced pulmonary injury in rats PMID: 21436609
5. KGF promotes preadipocyte proliferation by an autocrine mechanism that involves activation of the PI3K/Akt signaling pathway PMID: 20069574
6. Keratinocyte growth factor may promote alveolar epithelial repair through several mechanisms involved in cell migration PMID: 12581741
7. Decreased gene expression of FGF-7 in the rat hypoplastic lung suggests that pulmonary hypoplasia in nitrofen-induced congenital diaphragmatic hernia rat may be caused by reduced synthesis of FGF-7 during lung morphogenesis. PMID: 12861530
8. KGF stimulates lipogenesis in alveolar type II cells by a coordinated expression of lipogenic enzymes and transport proteins regulated by C/EBP isoforms and SREBP-1c PMID: 12865412
9. stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3 PMID: 14506240
10. Exogenous administered KGF cDNA causes increases in IGF-I, IGF-BP3, FGF, and collagen IV and decreases TGF-beta concentration. KGF gene transfer accelerates wound healing without causing an increase in collagen I or III. PMID: 17292422
11. KGF improves alterations of lung permeability and bronchial epithelium in allergic rats PMID: 17392324
12. retention of the AT2 cell phenotype by KGF involves c-Jun and suggest that activation of c-Jun kinase may be an important determinant of maintenance of AT2 cell phenotype PMID: 17872496
13. FGF-7, most likely through its effect on the vascular bed, is required for normal early postnatal alveolar formation from secondary crests. PMID: 18091341
14. the effects of KGF on duct cell proliferation are mediated by the MEK-ERK1/2 pathway, while the KGF-induced cell differentiation is mediated by the PI3K/AKT pathway PMID: 19266047
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蛋白家族：

Heparin-binding growth factors family
數(shù)據(jù)庫鏈接：

KEGG: rno:29348

STRING: 10116.ENSRNOP00000012700

UniGene: Rn.98842

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.