Rat Gremlin ELISA Kit

文獻引用 (1) 說明書下載

中文名稱：

大鼠骨形態形成蛋白拮抗蛋白酶聯免疫試劑盒
貨號：

CSB-E17688r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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大鼠骨形態形成蛋白拮抗蛋白酶聯免疫試劑盒（CSB-E17688r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的GREM1含量。GREM1作為靶點，在機體胚胎發育、組織修復等過程中發揮重要作用。研究發現它能通過調控BMP信號通路等影響細胞的增殖、分化和凋亡，與多種疾病如腫瘤、骨疾病等發生發展相關，對其深入研究有助于開發新的治療策略和藥物。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于體外培養細胞模型、動物實驗樣本中BMP拮抗蛋白的定量分析，可支持骨骼生物學研究、信號通路調控機制探索以及相關疾病的分子機制解析，為藥物干預效果評估或病理模型構建提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Grem1 ELISA Kit; Cktsf1b1 ELISA Kit; Drm ELISA Kit; Gremlin-1 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; BMP antagonist 1 ELISA Kit; Down-regulated in Mos-transformed cells protein ELISA Kit
縮寫：

GREM1
Uniprot No.：

O35793
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat gremlin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-109
1:2	Average %	96
	Range %	94-98
1:4	Average %	89
	Range %	83-95
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of rat gremlin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	99-104
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.210	2.222	2.216	2.094
50	1.798	1.844	1.821	1.699
25	1.432	1.503	1.468	1.346
12.5	1.053	1.062	1.058	0.936
6.25	0.751	0.722	0.737	0.615
3.12	0.391	0.411	0.401	0.279
1.56	0.258	0.266	0.262	0.140
0	0.121	0.123	0.122

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Angiogenic and Fibrogenic Dual-effect of Gremlin1 on Proliferative Diabetic Retinopathy EU CICERO,International journal of biological sciences,2024

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功能：

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed.
基因功能參考文獻：
1. gremlin1 promotes hepatic stellate cell activation and hepatic fibrogenesis through impairment of the balance between transforming growth factor beta and bone morphogenetic protein 7 signaling pathways. PMID: 27863390
2. The therapeutic mechanism of Danshao Huaxian capsule in hepatic fibrosis may be associated with inhibition of the expression of Gremlin and up-regulation of the expression of BMP-7. PMID: 25356047
3. GREM1 affects follicle transition in cultured ovaries, but is not normally present in primordial and early-stage developing follicles. PMID: 24614542
4. Notch pathway gene expression is elevated in diabetic nephropathy, co-incident with Gremlin, and may contribute to the pathogenesis of this dise PMID: 17980714
5. Gremlin plays a part in the development of pathological phenotypic changes of adult vascular smooth muscle cell. PMID: 18086474
6. The bone morphogenetic protein antagonist gremlin promotes vascular smooth muscle cell apoptosis. PMID: 19142012
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亞細胞定位：

Secreted.
蛋白家族：

DAN family
組織特異性：

Highly expressed in the brain, kidney, spleen, and testis and weakly expressed in the lung and liver. Predominantly expressed in differentiated cells as neurons in brain, type I cells in lung and globlet cells in intestine.
數據庫鏈接：

KEGG: rno:50566

STRING: 10116.ENSRNOP00000035888

UniGene: Rn.42929

Rat Gremlin ELISA Kit

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