Rat Cytochrome c(CYCS) ELISA kit

Rat Cytochrome c(CYCS) ELISA kit

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中文名稱：

大鼠細(xì)胞色素C(CYCS)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL006328RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠細(xì)胞色素C(CYCS)酶聯(lián)免疫試劑盒（CSB-EL006328RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CYCS含量。CYCS 即細(xì)胞色素 c，是位于線粒體內(nèi)膜外側(cè)的水溶性蛋白。它在細(xì)胞呼吸的電子傳遞鏈中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)，其從線粒體釋放到細(xì)胞質(zhì)會(huì)激活凋亡相關(guān)蛋白，進(jìn)而啟動(dòng)細(xì)胞凋亡機(jī)制，在細(xì)胞死亡調(diào)控等研究領(lǐng)域備受關(guān)注。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于大鼠模型中CYCS相關(guān)的基礎(chǔ)研究，例如線粒體功能評(píng)估、凋亡信號(hào)通路探索、藥物誘導(dǎo)的細(xì)胞損傷研究或心血管疾病模型的分子機(jī)制分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

CycsCytochrome c ELISA Kit; somatic ELISA Kit
縮寫：

CYCS
Uniprot No.：

P62898
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cell Biology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	89
	Range %	85-96
1:4	Average %	92
	Range %	90-95
1:8	Average %	91
	Range %	87-96

回收率：

The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	90	84-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.581	2.458	2.520	2.427
2500	2.234	2.186	2.210	2.117
1250	1.638	1.621	1.630	1.537
625	1.103	1.097	1.100	1.007
312	0.692	0.672	0.682	0.589
156	0.393	0.386	0.390	0.297
78	0.236	0.223	0.230	0.137
0	0.094	0.092	0.093

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
亞細(xì)胞定位：

Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
蛋白家族：

Cytochrome c family
組織特異性：

Found in embryos and in adult liver and heart.
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:100363502

STRING: 10116.ENSRNOP00000041521

UniGene: Rn.161841

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.