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中文名稱：大鼠組織蛋白酶K(cath-K)酶聯免疫試劑盒
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貨號：CSB-E09439r
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規格：96T/48T
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價格：￥3900/￥2500
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其他：
產品詳情
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產品描述：大鼠組織蛋白酶K(cath-K)酶聯免疫試劑盒（CSB-E09439r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTSK含量。CTSK（組織蛋白酶K）是一種在骨重塑過程中起關鍵作用的酶，主要在破骨細胞中表達。其研究機制涉及降解骨細胞外基質，促進骨吸收。CTSK在多種癌癥中表達，可能促進腫瘤細胞增殖、侵襲和遷移。研究CTSK有助于開發新的治療策略。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于大鼠骨代謝研究、骨骼疾病動物模型評估及骨靶向藥物作用機制探索等科研領域，可為骨生物學、病理機制研究及藥物開發提供穩定可靠的定量分析支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 104 Range % 100-107 1:2 Average % 99 Range % 95-103 1:4 Average % 85 Range % 80-90 1:8 Average % 107 Range % 103-110 -
回收率：
The recovery of rat cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 86 83-89 EDTA plasma (n=4) 95 90-100 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.801 2.765 2.783 2.682 500 2.342 2.204 2.273 2.172 250 1.343 1.406 1.375 1.274 125 0.679 0.701 0.690 0.589 62.5 0.399 0.405 0.402 0.301 31.2 0.251 0.242 0.247 0.146 15.6 0.166 0.164 0.165 0.064 0 0.102 0.100 0.101 -
數據處理：
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貨期：3-5 working days
引用文獻
- Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats H Liu,Phytother Res,2020
- Early changes in serum osteocalcin and body weight are predictive of implant fixation in a rat model of implant loosening Wilson B M, et al,Journal of Orthopaedic Research,2019
- Research article Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and trabecular bone loss Azza M. El-Wakf,Journal of Innovations in Pharmaceutical and Biological Sciences,2019
產品評價
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靶點詳情
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功能：Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
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基因功能參考文獻：
- the present study identified icariin as a novel therapeutic agent in DXM-induced cartilage injury in rats, and revealed that the activation of miR-206 targeting of cathepsin K may be responsible for the chondroprotective efficacy of icariin PMID: 29207029
- cathepsin K, in both its secreted and lysosomal forms, may play a role in the degradation of Sertoli cell residual bodies PMID: 14585819
- In conclusion, according to its ability to modulate hypotensive effects of kinins, we propose that cathepsin K is a kininase of biological relevance. PMID: 17181996
- Comparison of the S2 site between rat and human cathepsin K sequences indicated that two S2 residues at Ser134 and Val160 in rat are varied to Ala and Leu, respectively, in the human enzyme. PMID: 18664521
- The expression pattern of Ctsk in osteoclasts in physiological root resorption in the rat molar was determined. PMID: 18974601
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亞細胞定位：Lysosome. Secreted. Apical cell membrane; Peripheral membrane protein; Extracellular side.
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蛋白家族：Peptidase C1 family
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數據庫鏈接：
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