Rat Carcinoembryonic Antign,CEA ELISA Kit

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產品詳情

產品描述：

大鼠癌胚抗原(CEA)酶聯免疫試劑盒（CSB-E13926r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CEA含量。癌胚抗原（CEA）是一種存在于粘膜細胞中的糖蛋白，正常情況下在嬰幼兒和成人粘膜細胞中存在。在癌癥患者中，癌胚抗原的表達會增加，因此常作為腫瘤標志物。CEA的研究機制涉及其在細胞粘附、免疫反應、失巢凋亡和肝轉移中的作用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，癌癥機制研究、實驗動物模型中的腫瘤進展監測、體外研究腫瘤微環境、評估抗癌藥物干預效果、篩選潛在生物標志物、腫瘤生物學研究、藥理毒理實驗、基礎醫學探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

CEA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	86	80-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.