Rat Calcitonin,CT ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠降鈣素(CT)酶聯免疫試劑盒（CSB-E05132r）檢測血清、血漿、組織勻漿樣本中的CALCA含量。CALCA 是重要靶點。它在人體生理過程有諸多參與。對其研究機制主要從基因表達調控等方面探究，目的是明確它在疾病發生發展中的作用，為開發針對該靶點的藥物、開展精準治療等奠定基礎，有望推動相關疾病治療的進步。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，大鼠模型中的鈣代謝調控機制探索、骨骼疾病動物模型評估、甲狀腺相關腫瘤分子機制研究等實驗場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CALCA Calcitonin
縮寫：

CALCA
Uniprot No.：

P01257
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
Range %	87-101
1:2	Average %	88
Range %	80-95
1:4	Average %	95
Range %	86-106
1:8	Average %	90
Range %	82-100

回收率：

The recovery of rat CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-99
EDTA plasma (n=4)	94	88-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
亞細胞定位：

Secreted.
蛋白家族：

Calcitonin family
數據庫鏈接：

STRING: 10116.ENSRNOP00000014948

UniGene: Rn.90085