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中文名稱：大鼠丙氨酸氨基轉移酶(ALT)酶聯免疫試劑盒
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貨號：CSB-E13024r
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：大鼠丙氨酸氨基轉移酶(ALT)酶聯免疫試劑盒（CSB-E13024r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的GPT含量。試劑盒檢測范圍為3.12 mIU/mL-200 mIU/mL，該產品適用于實驗室中肝毒性評估、藥物代謝研究、疾病動物模型建立等場景，例如可通過檢測不同處理條件下肝組織勻漿或血清中的ALT水平變化，評估化學物質、藥物或病理狀態對肝臟功能的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Gpt ELISA Kit; Aat1 ELISA Kit; Gpt1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; ALT1 ELISA Kit; EC 2.6.1.2 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; GPT 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：3.12 mIU/mL-200 mIU/mL
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靈敏度：0.78 mIU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Metabolism
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 80-92 1:2 Average % 98 Range % 91-105 1:4 Average % 100 Range % 92-110 1:8 Average % 94 Range % 86-98 -
回收率：
The recovery of rat ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 96 90-100 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mIU/ml OD1 OD2 Average Corrected 200 2.814 2.911 2.863 2.778 100 2.466 2.355 2.411 2.326 50 1.659 1.564 1.612 1.527 25 0.853 0.812 0.833 0.748 12.5 0.469 0.442 0.456 0.371 6.25 0.243 0.226 0.235 0.150 3.12 0.149 0.141 0.145 0.060 0 0.088 0.082 0.085 -
數據處理：
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貨期：3-5 working days
引用文獻
- Bioassay-Guided Isolation and Active Compounds Identification of the AntiDiabetic Fractions of Centaurea calcitrapa Extract and the Predicted Interaction Mechanism HM Kadhim, YM Kadhim, HA Fawzi, ZM Abdul Khalik,Molecules,2025
- The mood stabilizers lithium and valproate disrupt hepatic and intestinal farnesoid X receptor signalling and increase bile synthesis in the rat S Cussotto, AV Golubeva, AL Gallardo,Experimental Physiology,2025
- Hepatocyte activation and liver injury following cerebral ischemia promote HMGB1-mediated hepcidin upregulation in hepatocytes and regulation of systemic iron levels D Davaanyam, SI Seol, SA Oh, H Lee,Experimental & Molecular Medicine,2024
- Efficacy and safety of intra-articular-only meropenem after one-stage revision for treating Escherichia coli-induced periprosthetic joint infection in a rat model Y Li, S Wuermanbieke, F Wang, W Mu,Bone & Joint Research,2024
- Effect of Liv.52hb, Ganoderma Lucidum, Stc30, and Astaxanthin on Serum TNF-α And Liver Function Indices Following CCL4 Induced Hepatocellular Carcinoma in Albino Wistar Rats Model JT Johnson, EU Modo,International Journal of Research in Medical and Clinical Science,2024
- Intranasal delivery of sunitinib: A new therapeutic approach for targeting angiogenesis of glioblastoma E Seidkhani,Toxicology and applied pharmacology,2023
- Interplay Between Inflammatory-immune and Interleukin-17 Signalings Plays a Cardinal Role on Liver Ischemia-reperfusion Injury—Synergic Effect of IL-17Ab, Tacrolimus and ADMSCs on Rescuing the Liver Damage SF Ko,Stem cell reviews and reports,2023
- Intra-wound versus systemic vancomycin for preventing surgical site infection induced by methicillin-resistant S. aureus after spinal implant surgery in a rat model J Wei,Journal of orthopaedic surgery and research,2023
- Upper gut heat shock proteins HSP70 and GRP78 promote insulin resistance, hyperglycemia, and non-alcoholic steatohepatitis G Angelini,Nature communications,2022
- Intra-wound vancomycin powder for the eradication of periprosthetic joint infection after debridement and implant exchange: experimental study in a rat model J Wei,BMC Microbiology,2021
- Cadmium chloride induces non-alcoholic fatty liver disease in rats by stimulating miR-34a/SIRT1/FXR/p53 axis AS Alshehri,Science of The Total Environment,2021
- RNA-sequencing analysis of the effect of luteolin on methamphetamine-induced hepatotoxicity in rats: a preliminary study Qu D,PeerJ,2020
- The Protective Activity of Broccoli Alcoholic Extract Against Augmentin effects In white Albino Male Rats AL QARAGHULIA N H, et al,International Journal of Pharmaceutical Research,2019
- Lactulose attenuates METH-induced neurotoxicity by alleviating the impaired autophagy, stabilizing the perturbed antioxidant system and suppressing apoptosis in rat striatum Xie XL.et al,Toxicology Letters Volume 289, 1 June 2018, Pages 10,2018
- Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies Wang Q.et al,Food Chem Toxicol. ,2017
- Effects of dietary essential oil and enzyme supplementation on growth performance and gut health of broilers challenged by Clostridium perfringens Qiujuan Sun. et al,Animal Feed Science and Technology,2015
- Different roles of hepatic hypothermic ischemia and ischemic preconditioning in chemically induced hepatocarcinogenesis in rats Qingan Qi et al,Journal of Surgical Research,2014
- Ameliorative potential of stem bromelain on lead-induced toxicity in Wistar rats Al-Otaibi WR. et al,Acta Biol Hung,2015
產品評價
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相關問答
■ 常見問題解答
Q:
Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since, we cannot collect more than 150 μL of blood from mice, we can use only 50 μL serum for ELISA. Will it fall within the detectable range ?
A:
We are glad to inform you the following details:
CSB-E13024r,Rat Alanine aminotransferase(ALT) ELISA Kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
CSB-E13024r,Rat Alanine aminotransferase(ALT) ELISA Kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
Q:
CSB-E13024r
1. In what units, the final data will be represented ? i.e in enzyme activity (IU/L) or
enzyme levels (ng/mg of protein).
2. The reference articles (Article attached) given in the website has mentioned the result
in U/I. What is this unit and how this unit is derived ?
3. Kindly mention the species in which the primary antibody (used in this kit) was raised.
A:
Thanks for your inquiry.
Pls check our answer:
The kit csb-e13024r kit uses two antibodies, mouse monoclonal antibody pre-coated in the plate and goat polyclonal antibody with biotin label. Both of the antibodies are use mIU/mL as the unit.
The kit is quantitatively detected the content of Alanine aminotransferase(ALT).
As for the published reference, pls check this link: https://www.cusabio.com/manage/index.php?action=class&todo=content&m=248&page=1&year=1&code=CSB-E13024r
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.
Pls check our answer:
The kit csb-e13024r kit uses two antibodies, mouse monoclonal antibody pre-coated in the plate and goat polyclonal antibody with biotin label. Both of the antibodies are use mIU/mL as the unit.
The kit is quantitatively detected the content of Alanine aminotransferase(ALT).
As for the published reference, pls check this link: https://www.cusabio.com/manage/index.php?action=class&todo=content&m=248&page=1&year=1&code=CSB-E13024r
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.
靶點詳情
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功能：Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
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基因功能參考文獻：
- Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
- Data suggest that both Alt1 and Alt2 are induced in liver during gluconeogenesis and in diabetes (Goto-Kakizaki rats); in hepatocytes, both Alt1 and Alt2 are induced by glucocorticoid dexamethasone and are suppressed by insulin. PMID: 25865565
- Although ALT and GMT activities and TNF-alpha and IL-6 concentrations were higher in male rats histopathological findings have shown slightly more advanced fibrotic and inflammatory changes in female rats. PMID: 17582939
- Report the early effects of short-term dexamethasone administration on hepatic and serum alanine aminotransferase in the rat. PMID: 18850354
- Protein levels are significantly elevated and correlate with activity, providing the molecular basis for the elevated total serum enzyme activity in liver toxicity. PMID: 19085960
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亞細胞定位：Cytoplasm.
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蛋白家族：Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
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組織特異性：Liver, heart, skeletal muscle, etc.
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數據庫鏈接：
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