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中文名稱：大鼠活化素A(ACV-A)酶聯免疫試劑盒
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貨號：CSB-E04503r
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規格：96T/48T
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價格：￥3900/￥2500
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其他：
產品詳情
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產品描述：大鼠活化素A(ACV-A)酶聯免疫試劑盒（CSB-E04503r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACV-A含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于研究ACV-A在生殖生物學、免疫調控機制、代謝性疾病模型中的表達動態，或用于評估藥物干預、基因編輯等實驗條件下ACV-A的分泌水平變化，為相關領域的分子機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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縮寫：ACV-A
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
250.287
251.377
254.271
255.277
253.524
253.271
SD
0.029
0.030
0.034
0.044
0.042
0.045
CV(%)
3.76
4.61
4.83
6.52
6.46
7.08
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
90
Range %
87-93
1:2
Average %
101
Range %
96-108
1:4
Average %
87
Range %
84-91
1:8
Average %
100
Range %
95-105
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回收率：
The recovery of rat ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
99
95-104
EDTA plasma (n=4)
87
83-90
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標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.582
2.601
2.592
2.474
500
1.959
1.869
1.914
1.796
250
1.301
1.298
1.300
1.182
125
0.823
0.831
0.827
0.709
62.5
0.498
0.501
0.500
0.382
31.2
0.325
0.336
0.331
0.213
15.6
0.237
0.241
0.239
0.121
0
0.117
0.119
0.118
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數據處理：
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貨期：3-5 working days
引用文獻
- Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model H Yue,Biomedicine & pharmacotherapy,2022
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