Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit
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中文名稱：兔子基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E06935Rb
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：兔子基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)酶聯(lián)免疫試劑盒（CSB-E06935Rb）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TIMP1含量。TIMP1即金屬蛋白酶組織抑制因子1，在細(xì)胞外基質(zhì)代謝、腫瘤生長(zhǎng)轉(zhuǎn)移等過程中扮演重要角色。研究機(jī)制方面，它可抑制基質(zhì)金屬蛋白酶活性，調(diào)節(jié)細(xì)胞增殖、凋亡等。當(dāng)前圍繞它在疾病診斷、治療靶點(diǎn)等方向開展諸多研究，有望為相關(guān)疾病干預(yù)提供新思路。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，可滿足體外實(shí)驗(yàn)中對(duì)TIMP - 1表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)需求，例如在細(xì)胞模型或動(dòng)物實(shí)驗(yàn)中探究炎癥反應(yīng)、組織重塑或疾病進(jìn)展相關(guān)機(jī)制，尤其適用于復(fù)雜生物樣本中TIMP - 1的科研級(jí)定量研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Oryctolagus cuniculus (Rabbit)
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樣本類型：serum, plasma, cell culture supernates , tissue homogenates
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檢測(cè)范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：7.8 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cardiovascular
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 90 Range % 85-97 1:200 Average % 102 Range % 98-108 1:400 Average % 98 Range % 94-102 1:800 Average % 88 Range % 84-93 -
回收率：
The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 92 88-96 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1000 0.086 0.084 0.085 500 0.145 0.141 0.143 250 0.245 0.238 0.242 125 0.392 0.413 0.403 62.5 0.725 0.727 0.726 31.2 0.963 0.944 0.954 15.6 1.154 1.179 1.167 0 1.734 1.662 1.698 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- The Use of Mesenchymal Stem Cells in the Complex Treatment of Kidney Tuberculosis (Experimental Study) AN Muraviov,Biomedicines,2022
- The Effect of Prostaglandin Analogues on the Ciliary Zonular Fibers of the Rabbit Crystalline Lens Hyung Bin Hwang.et al,Journal Current Eye Research,2018
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相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
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基因功能參考文獻(xiàn)：
- Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
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亞細(xì)胞定位：Secreted.
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蛋白家族：Protease inhibitor I35 (TIMP) family
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數(shù)據(jù)庫鏈接：
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