Rabbit luteinizing hormone (LH) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子黃體生成素（LH）酶聯免疫試劑盒（CSB-E17780Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白激素。其生理背景在于參與調節生殖系統，對女性排卵和男性睪酮合成起關鍵作用。研究機制上，主要通過與靶細胞上受體結合，激活信號通路，調控生殖相關基因表達與細胞功能。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，該產品可應用于兔繁殖生物學研究、激素水平動態監測、生殖疾病模型評估或藥物干預實驗中LH表達變化的分析，尤其適用于卵巢、垂體等組織局部LH表達水平的檢測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LHBLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
縮寫：

LH
Uniprot No.：

Q6IY74
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	90
	Range %	84-95
1:4	Average %	96
	Range %	89-102
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of rabbit LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-104
EDTA plasma (n=4)	100	94-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
基因功能參考文獻：
1. This study determined the cDNA sequences of the luteinizing hormone beta-subunit (LHB) from Japanese White (JW), New Zealand White (NZW), and Dutch-Belted (Dutch) rabbits, and we compared these LHB sequences with those of other mammals. PMID: 17094992
亞細胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數據庫鏈接：

KEGG: ocu:100009427

STRING: 9986.ENSOCUP00000025405

UniGene: Ocu.3341