Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit
-
中文名稱:兔載脂蛋白B100(Apo-B100)酶聯免疫試劑盒
-
貨號:CSB-E09805Rb
-
規格:96T/48T
-
價格:¥4200/¥3000
-
其他:
產品詳情
-
產品描述:兔載脂蛋白B100(Apo-B100)酶聯免疫試劑盒(CSB-E09805Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Apo-B100含量。Apo-B100是低密度脂蛋白(LDL)的主要結構蛋白,參與脂質和膽固醇代謝,與動脈粥樣硬化密切相關。研究顯示,Apo-B100通過冷凍電鏡、AlphaFold2和分子動力學方法解析其結構,揭示其與LDL受體(LDLR)的相互作用機制,為開發針對膽固醇代謝異常的新療法提供新視角。試劑盒檢測范圍為78 ng/mL-5000 ng/mL,靈敏度為19.5 ng/mL。適用于基礎科研中脂代謝機制研究、動脈粥樣硬化病理模型分析、藥物干預對脂蛋白合成影響的評估等方向,為心血管疾病相關動物實驗提供可靠的檢測工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
-
別名:APOBApolipoprotein B ELISA kit; Fragment ELISA kit
-
縮寫:Apo-B100
-
Uniprot No.:
-
種屬:Oryctolagus cuniculus (Rabbit)
-
樣本類型:serum, plasma, tissue homogenates
-
檢測范圍:78 ng/mL-5000 ng/mL
-
靈敏度:19.5 ng/mL
-
反應時間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領域:Cardiovascular
-
測定原理:quantitative
-
測定方法:Competitive
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 96 Range % 90-102 1:200 Average % 92 Range % 87-96 1:400 Average % 104 Range % 98-108 1:800 Average % 85 Range % 80-89 -
回收率:
The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 93-102 EDTA plasma (n=4) 87 82-92 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 5000 0.092 0.091 0.092 2500 0.146 0.138 0.142 1250 0.246 0.236 0.241 625 0.375 0.360 0.368 312 0.524 0.539 0.532 156 0.934 0.877 0.906 78 1.447 1.334 1.391 0 2.432 2.562 2.497 -
數據處理:
-
貨期:3-5 working days
相關產品
靶點詳情
-
最新研究進展:Apo-B100是由APOB基因編碼的蛋白質,是低密度脂蛋白(LDL)的主要成分之一。最新研究表明,Apo-B100在冠心病的發病中扮演了重要的角色。研究人員發現,Apo-B100水平升高與冠心病的風險增加有關。此外,一些研究還表明,調節Apo-B100的水平可以改善血脂和血糖代謝,從而降低心血管疾病的風險。
-
功能:Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
-
亞細胞定位:Cytoplasm. Secreted. Lipid droplet.
-
組織特異性:Detected in liver (at protein level). Highly expressed in liver. Detected at lower levels in jejunum, duodenum and kidney.
-
數據庫鏈接:
STRING: 9986.ENSOCUP00000004631
UniGene: Ocu.1828
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-