Rabbit Tumor necrosis factor α,TNFα ELISA Kit

Rabbit Tumor necrosis factor α,TNFα ELISA Kit

產品詳情

引用文獻

產品評價

靶點詳情

產品詳情

引用文獻

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息

文獻引用 (36) 評價 (4) 說明書下載

中文名稱：

兔子腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
貨號：

CSB-E06998Rb
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品描述：

兔子腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E06998Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TNF含量。TNF（腫瘤壞死因子）是一種重要的細胞因子，在免疫調節、炎癥反應等生理病理過程中發揮關鍵作用。研究表明，TNF可激活多條信號通路，介導細胞存活、凋亡、炎癥因子釋放等。異常的TNF表達與多種疾病如類風濕關節炎等密切相關，是藥物研發熱門靶點。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，本試劑盒適用于兔模型在病原體感染、炎癥機制解析、藥物干預效果評估等基礎科研領域的研究需求，為探索TNF-α在免疫應答中的動態變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
縮寫：

TNF
Uniprot No.：

P04924
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	98
	Range %	92-102
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	92	87-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.905	2.899	2.902	2.789
2500	2.366	2.356	2.361	2.248
1250	1.639	1.604	1.622	1.509
625	0.971	0.939	0.955	0.842
312	0.488	0.456	0.472	0.359
156	0.275	0.259	0.267	0.154
78	0.179	0.171	0.175	0.062
0	0.116	0.110	0.113

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Противовоспалительное действие и внутриглазная кинетика бромфенака при переднем увеите в зависимости от состава топической лекарственной формы ЕВ Блинова, ПА Лябушев, ЕА Литвин,Российский офтальмологический журнал,2024
Cationic homopolypeptides: A versatile tool to design multifunctional antimicrobial nanocoatings L Kocgozlu, A Mutschler, L Tallet, C Calligaro,Materials Today Bio,2024
γ-Cyclodextrin-based polypseudorotaxane hydrogels for ophthalmic delivery of flurbiprofen to treat anterior uveitis G Fang,Carbohydrate polymers,2023
Use of the improved tug-of-war acupuncture for promoting cartilage repair by inducing macrophage polarization in knee osteoarthritis P Tangseefa,Heliyon,2024
Atorvastatin liposomes in a 3D-printed polymer film: a repurposing approach for local treatment of oral candidiasis EM Nour,Drug delivery and translational research,2023
Dietary addition of Humulus scandens improved the intestinal barrier in rabbits G Liu,Journal of animal science,2023
Effects of alginates on the growth, haematological, immunity, antioxidant and pro-inflammatory responses of rabbits under high temperature SAM Saghir,Research in veterinary science,2023
In Vivo Pathogenicity of Methicillin-Susceptible Staphylococcus aureus Strains Carrying Panton–Valentine Leukocidin Gene F Yildirim,Life,2022
Quercetin ameliorated remote myocardial injury induced by renal ischemia/reperfusion in rats: Role of Rho-kinase and hydrogen sulfide Shaimaa S El-Saye,Life Sciences,2021
CRISPR/Cas9-Mediated Knockout of APOC3 Stabilizes Plasma Lipids and Inhibits Atherosclerosis in Rabbits Y Zha,Researchsquare,2021
Skin fibroblasts' alteration after Acupuncture on LI11 in Rabbits with bacteria endotoxin induced fever W Fan,Acupuncture & Electro-Therapeutics Research,2021
Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits H Zhao,czech journal of animal science,2020
Mesenchymal stem cells significantly improved treatment effects of Linezolid on severe pneumonia in a rabbit model Kong D, et al,Bioscience Reports,2019
Chondroprotective and antiarthritic effects of daphnetin used in vitro and in vivo osteoarthritis models Xiaohan Zhang, et al,Life Sciences,2019
Effect of febuxostat on biochemical parameters of hyperlipidemia induced by a high-fat diet in rabbits Heikal MM,Canadian Journal of Physiology and Pharmacology,2019
Evaluation of comparative soft tissue response to bone void fillers with antibiotics in a rabbit intramuscular model Oliver RA, et al,Journal of Biomaterials Applications,2019
Effect of febuxostat on biochemical parameters of high fat diet-induced hyperlipidemia in rabbits Heikal M, et al,Canadian journal of physiology and pharmacology,2019
Phenolic-Rich Baccaurea angulata Modulates Inflammatory Biomarkers of Atherosclerosis Muhammad Ibrahim, et al,Journal of Nutrition and Metabolism,2018
Experimental Study of Phlebitis Ointment Administration in Acute Superficial Thrombophlebitis Guangzong Li.et al,EVID-BASED COMPL ALT,2018
Biochemical and Apoptotic Biomarkers of Experimentally Induced Traumatic Brain Injury: In Relation to Time since Death Amany Abdel-Rahman Mohamed.et al,Beni-Suef University Journal of Basic and Applied Sciences,2018
Biochemical and Apoptotic Biomarkers as Indicators of Time Elapsed Since Death in Experimentally Induced Traumatic Brain Injury Amany Abdel-Rahman Mohamed.et al, SM J Forensic Res Criminol 1.2,2017
Treatment evaluation of Wharton’s jelly-derived mesenchymal stem cells using a chronic salpingitis model: an animal experiment Li Z.et al,Stem Cell Res Ther,2017
Poly(Lactic Acid) Blends with Poly(Trimethylene Carbonate) as Biodegradable Medical Adhesive Material Zhang S.et al,Int. J. Mol. Sci,2017
EFFECTS OF AMLODIPINE AGAINST CISPLATIN-INDUCED NEPHROTOXICITY IN RABBITS Ahmed Abu Raghif.et al,/,2015
Probucol prevents atrial remodeling by inhibiting oxidative stress and TNF-α/NF-κB/TGF-β signal transduction pathway in alloxan-induced diabetic rabbits. Fu H.et al,J Cardiovasc Electrophysiol.,2015
Involvement of AMPK/SIRT1 pathway in anti-allodynic effect of troxerutin in CCI-induced neuropathic pain Gui Y. et al,Eur J Pharmacol,2015
Effects of aqueous thyme extract against cisplatin induced nephrotoxicity in rabbits Ahmed Rhahma Abu-Raghif. et al,Int. J. Pharm. Sci. Rev. Res.,2015
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue Oliver RA.et al,PLoS One.,2015
Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair Chen, Guofei et al,Journal of Biomaterials and Tissue Engineering,2015
Clinical Features of Chronic Trypanosomosis in Rabbits Experimentally Infected with the Malaysian Isolate ofTrypanosoma evansi Yasameen et al,IOSR-JAVS,2015
Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone Li Y. et al,Osteoarthritis Cartilage,2015
Cell factory-derived bioactive molecules with polymeric cryogel scaffold enhance the repair of subchondral cartilage defect in rabbits Gupta A. et al,J Tissue Eng Regen Med,2015
Dihydromyricetin improves glucose and lipid metabolism and exerts anti-inflammatory effects in nonalcoholic fatty liver disease: A randomized controlled trial Chen S. et al,Pharmacol Res.,2015
Assessing the Systemic Toxicity in Rabbits after Sub Acute Exposure to Ocular Irritant Chemicals Reshma CS et al,Toxicol Res,2015
Hematologic and immunological characteristics of Henoch-Schonlein purpura in rat and rabbit models induced with ovalbumin based on type III ? Li Y et al,Sci Rep,2015
The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol Hacibeyoglu G et al,Fundam Clin Pharmacol,2014

收起更多

平均分:

5.0分 - 4 個評價

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒有稀釋

產品評價：我用CSB-E06998Rb檢測模型組術前，術中30min-2h，術后24h血漿，四參數模型擬合出來的R2為0.99868（詳見圖片），標準曲線符合預期。0-2h的TNF-a水平符合升高趨勢，經過文獻查詢，該模型需要6h才能檢測出大量升高的TNF-a炎癥因子水平，后續會測量6h及以上的炎癥因子水平。課題組師兄推薦，有兔子種屬，且價格實惠，測試結果準確。

By 陳老師

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒有稀釋

產品評價：我用CSB-E06998Rb測模型組術前，術中30min-2h，術后24h血漿，標準曲線用四參數模型擬合出來的R2為0.99783（詳見圖片），標準曲線做了兩列，符合預期且OD值在0至3.6范圍內，且得到的數據符合預期趨勢。該試劑盒測試穩定，均能顯色。

By 陳老師

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒有稀釋

產品評價：我用CSB-E06998Rb測組蛋白注射模型組術前，術中30min-2h，術后24h血漿，標準曲線用四參數模型擬合出來的R2僅為0.68，而直線擬合出來R2為0.9996（數據和擬合曲線方程詳見圖片），標準曲線做了兩列，符合預期且OD值在0至3.2范圍內，且得到的數據符合預期趨勢，術前測出來約600-800pg/ml。術中逐漸升高至1400pg/ml，術后24h降至1000 pg/ml左右，該試劑盒測試穩定，做了兩個復孔，誤差較小但均能顯色，且兔子個體差異大，因此我準備用Fold Change來表示，該數據準備用來發文章。

By 陳老師

樣品類型：血漿（抗凝劑）水蛭素

樣品信息：其他兔

稀釋比：沒有稀釋

產品評價：我用CSB-E06998Rb測正常血漿，組蛋白刺激以及材料和血漿共孵育，標準曲線用四參數模型擬合出來的R2為0.99814（詳見圖片），標準曲線做了兩列，標曲符合預期且OD值在0至1.6范圍內，且得到的數據符合預期趨勢，組蛋白刺激后炎癥因子釋放約為正常組的2倍。該試劑盒測試穩定，均能顯色。

By 陳老師

展示更多評論 >>

最新研究進展：

腫瘤壞死因子（TNF）是一種細胞因子，在免疫調節、炎癥和細胞凋亡等生物學過程中發揮重要作用。近年來的研究表明，TNF與各種疾病的發病機制有關，例如炎癥性疾病、腫瘤、自身免疫性疾病等。一些新的抗TNF治療藥物也被開發出來，已經用于治療類風濕性關節炎、克羅恩病等疾病，并獲得了一定的療效。
功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻：
1. TUNEL staining was positively correlated with TNF-a protein expression. Our findings suggest that apoptosis can be induced in the vocal fold epithelium after 120min of modal intensity phonation. In contrast, shorter durations of vibration exposure do not result in apoptosis signaling. PMID: 27577014
2. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases. PMID: 26125840
3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
4. The TNFalpha-evoked Cl- current. PMID: 24084720
5. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
6. Hypercapnia increased expression of TNFa and decreased expression of NFKB in acute lung injury models. PMID: 21158126
7. In the early stages of myocardial ischemia, bone marrow stem cells are mobilized and home to ischemic myocardium with a concomitant increase in expression of cytokines VEGF and TNFalpha. PMID: 20624659
8. Ammonium perchlorate can increase gene expressions of types I, III collagens, TGF-beta(1) and TNF-alpha in lung of rabbits. PMID: 20937628
9. TNFalpha may either be directly or indirectly involved in vascular damage following an embolic stroke. Moreover, TNFalpha may mediate some of the detrimental effects of tPA on the vascular compartment PMID: 17673188
顯示更多

收起更多
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: ocu:100009088

STRING: 9986.ENSOCUP00000005787

UniGene: Ocu.1971

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.