Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子抗酒石酸酸性磷酸酶5b(TRAP5b)酶聯(lián)免疫試劑盒（CSB-E15043Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TRAP5b含量。試劑盒檢測范圍為78 μU/mL-5000 μU/mL，適用于科研領(lǐng)域中對骨代謝相關(guān)疾病（如骨質(zhì)疏松、骨轉(zhuǎn)移癌、風濕性關(guān)節(jié)炎等）的機制研究，也可用于藥物開發(fā)中破骨細胞活性調(diào)節(jié)劑的體外效果評估。通過檢測樣本中TRAP5b的表達水平，研究者可量化分析骨吸收活性變化，為骨骼系統(tǒng)疾病模型構(gòu)建、病理機制探索及治療策略優(yōu)化提供實驗依據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ACP5 ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
縮寫：

TRAP5b
Uniprot No.：

O97860
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 μU/mL-5000 μU/mL
靈敏度：

19.5 μU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-94
1:4	Average %	97
	Range %	87-101
1:8	Average %	91
	Range %	84-95

回收率：

The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-96
EDTA plasma (n=4)	103	93-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.