Rabbit Pulmonary surfactant-associated protein B(SFTPB) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔肺表面活性物質相關蛋白B(SFTPB)酶聯免疫試劑盒（CSB-EL021173RB）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPB含量。試劑盒檢測范圍為1.87 ng/mL-120 ng/mL，該產品適用于呼吸生理學研究，可探究SFTPB在肺損傷修復、感染性肺炎或遺傳性肺泡蛋白沉積癥等動物模型中的動態變化，也可用于評估肺表面活性物質代謝調控機制或藥物干預效果。支持血清、血漿直接檢測及肺組織等勻漿樣本處理，滿足不同實驗設計的科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
縮寫：

SFTPB
Uniprot No.：

P15285
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.87 ng/mL-120 ng/mL
靈敏度：

0.46 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	91-99
1:4	Average %	94
	Range %	89-98
1:8	Average %	89
	Range %	86-96

回收率：

The recovery of rabbit SFTPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	88	82-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
亞細胞定位：

Secreted, extracellular space, surface film.
數據庫鏈接：

KEGG: ocu:100009194

STRING: 9986.ENSOCUP00000025658

UniGene: Ocu.1963