Rabbit Pulmonary surfactant-associated protein A(SFTPA1) ELISA kit
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中文名稱：兔肺表面活性物質(zhì)相關(guān)蛋白A(SFTPA1)酶聯(lián)免疫試劑盒
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貨號：CSB-EL021168RB
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規(guī)格：96T/48T
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價格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：兔肺表面活性物質(zhì)相關(guān)蛋白A(SFTPA1)酶聯(lián)免疫試劑盒（CSB-EL021168RB）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPA1含量。SFTPA1，即肺表面活性物質(zhì)相關(guān)蛋白A，是一種在肺泡II型上皮細(xì)胞中合成的蛋白質(zhì)，主要功能是降低肺泡表面張力，維持肺泡穩(wěn)定性。其研究涉及肺泡生理、病理及疾病治療等領(lǐng)域。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研領(lǐng)域中對兔呼吸道疾病模型的機(jī)制研究，如評估肺損傷修復(fù)過程中 SFTPA1 的動態(tài)變化，探究感染或炎癥狀態(tài)下肺泡蛋白的調(diào)控規(guī)律，或用于藥物干預(yù)實(shí)驗(yàn)中生物標(biāo)志物的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：SFTPA1 ELISA Kit; SFTP1 ELISA Kit; SFTPA ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
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縮寫：SFTPA1
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Uniprot No.：
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種屬：Oryctolagus cuniculus (Rabbit)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：1.56 ng/mL-100 ng/mL
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靈敏度：0.39 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 82-96 1:2 Average % 107 Range % 90-111 1:4 Average % 90 Range % 85-94 1:8 Average % 91 Range % 85-95 -
回收率：
The recovery of rabbit SFTPA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 82-95 EDTA plasma (n=4) 99 90-105 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 100 2.110 1.991 2.051 1.950 50 1.813 1.688 1.751 1.650 25 1.382 1.484 1.433 1.332 12.5 1.053 1.028 1.041 0.940 6.25 0.726 0.705 0.716 0.615 3.12 0.536 0.528 0.532 0.431 1.56 0.309 0.302 0.306 0.205 0 0.098 0.104 0.101 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Pathophysiological changes and injury markers for acute lung injury from blunt impact in infant rabbits K Wang,Frontiers in pediatrics,2024
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
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亞細(xì)胞定位：Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
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蛋白家族：SFTPA family
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數(shù)據(jù)庫鏈接：
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