Rabbit Krebs yon den Lundgen-6 (KL-6) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子Ⅱ型肺泡細(xì)胞表面抗原(KL-6)酶聯(lián)免疫試劑盒（CSB-E14112Rb）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的KL-6含量。KL-6，也稱為涎液化糖鏈抗原，是一種表達(dá)于II型肺泡上皮細(xì)胞的糖蛋白，被認(rèn)為是診斷間質(zhì)性肺疾?。↖LD）的準(zhǔn)確性最高的生物標(biāo)記物。其表達(dá)增多與肺泡上皮細(xì)胞增生和肺泡基底膜損傷有關(guān)，在ILD的早期診斷和病情評(píng)估中具有重要價(jià)值。試劑盒檢測(cè)范圍為50 IU/mL-800 IU/mL，支持科研人員在體外實(shí)驗(yàn)中定量評(píng)估肺損傷模型、藥物干預(yù)研究或疾病生物標(biāo)志物探索中的 KL - 6 動(dòng)態(tài)變化，為呼吸系統(tǒng)疾病的基礎(chǔ)研究提供靈敏可靠的工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

KL-6
Uniprot No.：
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

50 IU/mL-800 IU/mL
靈敏度：

25 IU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit KL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rabbit KL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	89	87-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.