Rabbit Interleukin 4,IL-4 ELISA Kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E06902Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是一種細胞因子，在免疫系統(tǒng)中扮演重要角色。它主要由Th2細胞等分泌，能促進B細胞增殖分化、調(diào)節(jié)免疫球蛋白類別轉(zhuǎn)換。在研究機制上，其通過與IL4受體結(jié)合激活下游信號通路，參與過敏、哮喘等疾病發(fā)生發(fā)展，是相關(guān)疾病治療的潛在靶點。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL，適用于動物模型中的Th2型免疫應(yīng)答研究、炎癥性疾病機制探索或感染過程中細胞因子動態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫：

IL4
Uniprot No.：

Q9MZR8
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 pg/mL-40 pg/mL
靈敏度：

0.156 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

標(biāo)準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
亞細胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫鏈接：

KEGG: ocu:100302454

STRING: 9986.ENSOCUP00000018901

UniGene: Ocu.2462