Rabbit Cortisol ELISA Kit

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產品詳情

產品描述：

兔子皮質醇(Cortisol)酶聯免疫試劑盒（CSB-E06956Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Cortisol含量。皮質醇，又稱壓力荷爾蒙，是調節身體對壓力反應的關鍵激素。其分泌受垂體和腎上腺控制，參與調節血壓、免疫和新陳代謝。研究皮質醇作為靶點，旨在了解其與壓力、疾病及生理過程的關系，以開發新的治療策略。試劑盒檢測范圍為62.5 pmol/mL-1000 pmol/mL，該產品適用于科研領域，如動物應激模型研究、藥物對腎上腺功能影響的評估，或生理病理狀態下皮質醇動態變化的分析，為神經內分泌學、藥理學及基礎醫學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

Cortisol
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pmol/mL-1000 pmol/mL
靈敏度：

62.5 pmol/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	81-97
1:2	Average %	89
	Range %	82-98
1:4	Average %	90
	Range %	85-100
1:8	Average %	95
	Range %	89-105

回收率：

The recovery of rabbit Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	95	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.