Rabbit Bone Alkaline Phosphatase (BALP) ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子骨堿性磷酸酶(BALP)酶聯免疫試劑盒（CSB-E15042Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的BALP含量。BALP即骨堿性磷酸酶，它是成骨細胞的表型標志物。在骨代謝中，成骨細胞分泌BALP參與骨基質的合成與礦化。研究通過檢測其水平來反映成骨細胞活性及骨形成狀況，在骨疾病如骨質疏松、佝僂病等的診斷與病情監測中有重要意義。試劑盒檢測范圍為0.156 mIU/mL-10 mIU/mL，本試劑盒適用于骨代謝相關動物模型研究，如骨質疏松癥機制探索、骨折愈合進程監測、骨再生藥物療效評估等科研場景，為體外定量分析提供穩定可靠的數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

BALP
Uniprot No.：

G1SLU6
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

0.156 mIU/mL-10 mIU/mL
靈敏度：

0.039 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-92
1:4	Average %	89
	Range %	85-94
1:8	Average %	98
	Range %	90-101

回收率：

The recovery of rabbit BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-108
EDTA plasma (n=4)	99	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.