Pig plasminogen activator inhibitor 1,PAI-1 ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬纖溶酶原激活物抑制因子1(PAI-1)酶聯免疫試劑盒（CSB-E06534p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPINE1含量。SERPINE1 即纖溶酶原激活物抑制劑 -1，它在多種生理和病理過程中起關鍵作用。其背景與凝血、纖維蛋白溶解等相關。研究機制方面，它可抑制纖溶酶原激活物，調節細胞遷移、增殖等，在腫瘤、心血管疾病等發生發展中有重要影響。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于心血管疾病模型、代謝綜合征研究、創傷修復機制探索等科研領域，為分析PAI-1在凝血 - 纖溶系統失衡、器官纖維化或腫瘤微環境中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

SERPINE1 ELISA Kit; PAI1 ELISA Kit; PLANH1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
縮寫：

SERPINE1
Uniprot No.：

P79335
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-93
1:2	Average %	100
	Range %	95-104
1:4	Average %	90
	Range %	85-94
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of pig PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	98	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
基因功能參考文獻：
1. In one pig population and in female pigs, SERPINE1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1) was significantly associated with muscling, growth and fat accretion and in male animals with meat quality. PMID: 22497544
亞細胞定位：

Secreted.
蛋白家族：

Serpin family
數據庫鏈接：

KEGG: ssc:396945

UniGene: Ssc.9781