Pig hemoglobin (Hb) ELISA Kit

說明書下載

產品詳情

產品描述：

豬血紅蛋白(Hb)酶聯免疫試劑盒（CSB-EQ027276PI）為競爭法ELISA試劑盒，定量檢測血清、血漿、RBC裂解物.樣本中的Hb含量。Hb即血紅蛋白，是紅細胞內運輸氧的特殊蛋白質。其與氧氣結合和釋放對維持機體氧供至關重要。在研究機制上，常關注其結構與功能改變，如與疾病相關的變異影響攜氧能力，也有基于它開發藥物改善氧運輸等方面的研究。試劑盒檢測范圍為0.625 μg/mL-40 μg/mL，適用于科研領域評估紅細胞功能及血紅蛋白代謝相關研究；可用于營養學研究中評估飼料中鐵元素吸收效率、生理學實驗中的貧血模型構建，或毒理學實驗中紅細胞損傷程度的分析，為實驗室提供穩定、高效的檢測方案，滿足多種樣本類型的血紅蛋白定量需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Hb
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, lysate for RBC.
檢測范圍：

0.625 μg/mL-40 μg/mL
靈敏度：

0.312 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-105
1:2	Average %	92
	Range %	86-100
1:4	Average %	95
	Range %	89-99
1:8	Average %	95
	Range %	87-105

回收率：

The recovery of pig Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
Plasma (n=5)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.